Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
How much formation of grana stacks is optimal?

Proposed period of release:
01/05/2014 to 31/12/2018

Name of the Institute(s) or Company(ies)
Umeå University, Department of Plant Physiology;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
thale cressbrassicaceaearabidopsisarabidopsis thaliana

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Overexpression of the "Curt1A"-protein. This protein infleunces the degree of "stacking" in the thylakoid membrane, specifically if the grana stacks become high and slim, or broad and low. This has a small, but measurable, effect on photosynthesis. In addition resistence markers (ampicillin for selection during bacterial cloning, hygromycin and kanamycin for selection in the plant.

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The "Curt1A"-genen from Arabidopsis driven by the 35S-promoter and NOS 3-end. A c-Myc tag at the end of the Curt1A-protein, (this does not affect its properties). This is done in a mutant backgrund where the Curt1A-protein is missing due to inactivation by a Ds element. Resistence markers: ampicillin, hygromycin and kanamycin.

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated T-DNA insertion

Experimental Release

1. Purpose of the release:
Basic research on photosynthesis

2. Geographical location of the site:
Umeå University, Sweden

3. Size of the site (m2):
Less than 2 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Only a selective disadvantage since phososyntheis is compromised. No effect on the environment.

Brief description of any measures taken for the management of risks:
Our previously established protocol for management of the site (daily inspections, measures to eliminate cross-pollination and seed dispersal, waste management etc.) make it highly unlikely that and transgenic material will spread. Although Arabidopsis is a more or less obligate self-pollinator - and Arabidopsis does not grow in the region and it is doubtful that it could hybridize with any species found in the region - we nevertheless cover the field to prevent insect-born cross-pollination. All material - soil, plants etc - from the experimental area is treated like trangenic material, removed and distructed.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
23/06/2014 00:00:00