Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Fast-growing hybrid aspens – what is the relation between groth in greenhouse and in the field?

Proposed period of release:
01/06/2010 to 31/12/2014

Name of the Institute(s) or Company(ies)
Umeå University, Department of Plant Physiology;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-T89

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application consists of 16 different gene constucts where 15 Populus genes and one Arabidopsis ortholog to a Populus gene has been
down- or upregulated. They have been selected since they result in increased growth in greenhouse experiments. Four different vectors are used 1) pK7GWIWG2(I), 2) pK2GW7, 3) pPCV812-LMX5-Gateway and 4) pPCV702.
Gener introduced from these are 1) p35S followed by gene of interest in two inverted copies with an intron in between, followed by a terminator T35S, neomycin phosphotransferase NPT-II driven by the Nos-promotorn 2) p35S followed by gene of interest and a terminator T35S, NPTII driven by Nos-promotor, 3) LMX5 promotorn followed by gene of interest and terminator ffom nopaline syntase (pANos), Hygromycin resistance gene driven by nopaline syntase promotor (pNos) and bla (*-lactamase) with replicon from pBR322 and 4) CaMV 35S promotor followed by gene of interest and terminator from nopaline syntase (pANos), NPT-II driven by nopaline syntase promotor (pNos) and bla (*-lactamase) with replicon from pBR322. The 17 Populus genes codes for 1) an LRR protein 2) a GRAS transcription factor, 3) a phosphatidyl serine synthase (PSS) 4) a WRKY transcription factor 5) a KNOTTED-like homeobox transcription factor 6, 7, 8 och 9) different WRKY transcription factors 10) a bHLH transciption factor 11) praboably a regulator of the gibberelin response 12) a WRKY transcription factor 13) a HD*GLABRA2 transkription factor 14) a SET-domain protein and 15) a WRKT transcription factor. The Arabidopsis-orthologeu codes for a GA20 oxidas, involved in the gibberelin biosyntheis

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
See 2.

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind.plooinated and with wind-dispersed seeds, and can also propapagate from suckers. To our knowledge, the particualr clone has never flowered in Sweden during at least 20 years of growth.

Experimental Release

1. Purpose of the release:
The aim is to see which if the 18 lines, that all show increased growth in the field, also grow better in the field. In addition, we will investiga if herbivore- or pathogen-interactions are modified. The particual clone (T89) is not useful for commerciall forestry in Sweden, so none of these lines are ained at being commercialized. However, if some genes give increased grwth without any penalty, they may later be used in other lines.

2. Geographical location of the site:
Arlösa gård, ca 5 km ONO Halmstad and Häckeberga gods, on Romeleåsen

3. Size of the site (m2):
Less than a hectar at each site

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
We hope that some of the lines will show good growth in the field .If so, this may lead to better-yielding tree varieties for fibre- or energy production

Brief description of any measures taken for the management of risks:
Regular inspection for early detection of possibel flowering. Buffer zone to surrounding forests

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Regular inspection for early detection of possibel flowering. Buffer zone to surrounding forests

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
29/04/2010 00:00:00