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Notification report

General information

Notification Number
B/SE/04/1101

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
16/02/2004

Title of the Project
Potato starch with increased amylose content

Proposed period of release:
01/04/2004 to 31/10/2008

Name of the Institute(s) or Company(ies)
Plant Science Sweden AB, Herman Ehles väg 2-4
SE-268 31 Svalöv
Sweden;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
potato	solanaceae	solanum	solanum tuberosum	tuberosum	P763 and P800

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- inhibition of starch branching enzymes be1 and be2, which yields a higher fraction of amylose in the starch through a reduced branching of amylopectin

- Marker gene AHAS (imidazolinone tolerance) as selection gene to identify transgenic cells in tissue culture

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
T-DNA borders, pTiT37, for incorporation into plant chromosome.Recombinant AHAS gene, A. thaliana, imidazolinone tolerance in plant material.Promoter and polyadenylation sequence from nopaline synthase gene, A. tumefaciens, gene regulation. Inverted repeat fragments of be1 and be2, S. tuberosum, inhibition of amylopectin biosynthesis.GBSS promoter, S. tuberosum, expression of inverted repeat fragments.

6. Brief description of the method used for the genetic modification:
A. tumefaciens mediated transformation of cut leaf discs from S. tuberosum.Binary vector system has been used.Transgenic tissue has been selected on medium containing imazamox. A. tumefaciens has been killed with Claforan.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
- Investigate expression and stability of modified traits
- Identify and eliminate morphologically divergent clones
- Investigate the agricultural and industrial value of the modified lines
- Produce potato tubers for laboratory analyses
- Produce seed potatoes for future trials

2. Geographical location of the site:
Landskrona, Halmstad, Kristianstad, Alvesta, Skara, Luleå och Robertsfors communities

3. Size of the site (m2):
Max 50 000 m2 in total

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Modified potato lines with AHAS as marker gene (imazamox tolerance gene) grown in field test 2002 and 2003.
Modified potato lines with inhibition of starch branching enzymes and thus increased amylose content in field tests yearly 1998-2003.
No impact on the environment or human health has been observed during these trials.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no reason to assume that the transgenic lines will behave in and interact with the environment differently compared to their mother varieties.

There are no experiences that imply that an altered carbohydrate composition or carbohydrate content could influence frost tolerance neither of plants nor tubers. Neither high starch content (starch potatoes) nor altered sugar content/composition (chip potatoes) have such effects. Earlier genetic modifications with increased amylose content have not been shown to influence the frost tolerance.

The introduced selection marker gene gives an increased tolerance to herbicides of imidazolinone type. This gives no selective advantage compared to other potatoes outside the agricultural habitat. As herbicides of imidazolinone type are not used in Sweden no selective advantage in cultivated fields is given either.

Brief description of any measures taken for the management of risks:
- isolation distance: new transformants: 20 m

- careful harvest to avoid volunteers

- transportation in specific vehicles, closed containers or double sacks

- adjusted crop rotation: new transformants: fallow field the following year, no potato crop for one additional year

- destruction of waste material

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
10/05/2004 00:00:00
Remarks: