Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Application for the release into the environment of potatoes with altered starch composition (II) according to the Genetically Modified Organisms Decree

Proposed period of release:
01/01/2008 to 31/12/2013

Name of the Institute(s) or Company(ies)
BASF Plant Science GmbH, Carl-Bosch-Strasse 38, D-67056 Ludwigshaven GERMANY;

3. Is the same GMPt release planned elsewhere in the Community?
Germany; Sweden; Czech Republic;

Has the same GMPt been notified elsewhere by the same notifier?

If yes, notification number(s):
B/CZ/05/642; B/CZ/06/02; B/DE/03/154; B/DE/04/162; B/DE/05/173; B/DE/06/183; B/NL/03/10; B/NL/05/05; B/SE/00/1019; B/SE/02/1104-CON; B/SE/04/7943;

Other notifications
Potato lines with increased amylopectin content based on constructs pAP2 or pAP4:
- B/SE/02/1104, B/SE/04/7943 (by Plant Science Sweden AB)
- B/DE/04/162, B/DE/05/173/B/DE/06/183
- B/NL/03/10, B/NL/05/05
- B/CZ/05/642, B/CZ/06/02

Potato lines comparable (i.e. transformed with a very similar construct) to the lines that are subjects of this application have been previously released in Sweden, the Netherlands, Germany and the Czech Republic. Reference is made to the following further notifications: B/SE/00/1019, B/DE/03/154, B/NL/03/10 and B/CZ/05/642. The very same lines were tested in the Netherlands in 2006 under notification B/NL/05/05

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumP800

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- Increased accumulation of amylopectin starch in tubers
- Marker gene ahas (tolerance to Imidazolinones) as selection gene to identify transgenic cells in tissue culture

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
-T-DNA borders, pTiT37, for incorporation into plant chromosome.
-Recombinant ahas gene, A. thaliana, imidazolinone tolerance in plant material.
-Promoter and terminator from nopaline synthase gene, terminator from octopine synthase gene, A. tumefaciens, gene regulation.
-Promoter of gbss gene, S. tuberosum, gene regulation
-the antisense or inverted repeat fragment of the gbss gene leads to a reduction of the amylose fraction in the starch of the potato tuber resulting in a concomitant increase in the levels of amylopectin

6. Brief description of the method used for the genetic modification:
Plasmid-derived DNA was introduced into the potato lines by Agrobacterium-mediated gene transfer technology. This is standard technology for potato transformation.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
- Evaluate the quality of starch composition under field conditions
- Investigate the general agricultural value of the modified lines
- Produce seed potatoes for future trials

2. Geographical location of the site:
One release site will be in the municipality of Borger-Odoorn and a second in the municipality of Lingewaard.

3. Size of the site (m2):
10.000 m2 per site maximally will be planted annually

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
There have been previous releases of potato lines transformed with the same constructs in several countries, including the Netherlands. No adverse impacts on the environment or human health have been recorded in any of the trials.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified potato lines contain gbss gene fragments either in antisense orientation or as inverted repeat. Their expression leads to a reduced amylose accumulation in tubers, resulting in a concomitant increase in amylopectin content. All greenhouse and field observations so far confirm that the behaviour of the modified lines is identical to that of the recipient plant material. Field trials with constructs containing the same gbss gene fragment have been conducted since 1993.
The ahas gene expressed in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture. This confers no selective advantage in the field since Imidazolinone herbicides are not approved for use on crops in NL and no field tolerance is expected in the potato plants. No difference with respect to persistence in agriculturally utilised habitats or invasiveness into natural habitats as compared to conventional potato varieties is expected. Through the measures that are taken during the release, distance from or absence of conventionally cultivated potatoes or wild species, the possibility of any gene transfer can be virtually ruled out. Even in the very improbable event that pollen were to be transferred to genetically unmodified potato plants, no consequences are to be expected, since potato propagation conventionally takes place via tubers and not via seeds. The interactions of the genetically modified potato line with non-target organisms and the effects resulting from this will be comparable to those with conventional potato varieties.

Brief description of any measures taken for the management of risks:
An isolation distance of 20 m to other potato varieties will be observed. Planting and harvesting equipment will be cleaned on site to prevent the dispersal of GM tubers. There will be no potato cultivation on the release area the year following the release. Potential volunteers will be monitored and removed according to conventional agricultural practice. During the release the trial site will be monitored at defined intervals.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
22/01/2008 00:00:00