Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Safety assessment of the release of transgenic crops spread of herbicide resistance genes from wheat.

Proposed period of release:
01/01/2003 to 31/12/2007

Name of the Institute(s) or Company(ies)
Instituto Nacional de Investigaciones Agrarias y Alimentarias INIA (MAPA), ;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

If yes, notification number(s):

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
wheatpoaceaetriticumtriticum aestivumDeganit

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
A gene from Pseudomonas putida coding for a dehalogenase degrading 2,2 dichloropropionic acid (2,2 DCPA) the active ingredient of herbicide Dalapon herbicide was transferred to wheat to confer dalapon resistance.

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:

6. Brief description of the method used for the genetic modification:
Biolistic transformation of embryogenic wheat calli.

Experimental Release

1. Purpose of the release:
The purpose of this release is to provide science based information on the possibility and probability of transgene introgression from wheat into related wild and weed species, to address biosafety implications of pollen movement and evaluating the impact (if any) of transgenes on farming activity and on the environment.

2. Geographical location of the site:
Experimental plots of the Departamento de Proteccion Vegetal (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria) Carretera de la Coruña Km 7,5. Madrid, Spain

3. Size of the site (m2):
400 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The impact is very low because the pollen has a life of twenty minutes and a potential of flow to pollinate other wheat of 4 meters (some grains were developed until 14 m at random at a very low rate)

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The potential environmental impact of these plants is quite inexistent in our conditions.
The herbicide dalapon is not used in Spain, it will be out of use in a near future in EU. A resistance gene is not a problem in the nature in absence of the selective herbicide.
The assay will be developed in a research experimental parcel located in a research institute in which the entrance is controlled. There is no cropping fields in the neighboring of the parcel that is surrounded by glasshouses and gardens.

Brief description of any measures taken for the management of risks:
The parcel will be covered with a net to avoid the presence of animals.
During the flowering period of the wheat the entire parcel and the zone surrounding it will be maintained free of wild and weeds grasses by manual and chemicals methods.
Harvest will be manual all the spikes collected. In the following autumn after emergency a herbicide total will be applied before new sowing.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Some plots of 1x1 m (the number of plots will be related to the number of hybrids obtained in crosses) of wheat will be shown with one F1 or F2 hybrid plant in the center of the plot. The same lay out will be used to obtain Aegilops x wheat and Agropyron x wheat hybrids, in this case, one Aegilops or Agropyron plant will be surrounded by Dalapon tolerant wheat. In both cases the aim is to study the intercrossing of these plants in field conditions. All plots will be isolated to avoid pollen flow.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
Not known