Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Evaluation of pollen spread of plastid located genes for the model plant Petunia hybrida under field conditions

Proposed period of release:
01/06/2009 to 31/12/2012

Name of the Institute(s) or Company(ies)
University of Rostock, ;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
petuniasolanaceaepetuniapetunia petunia x petunia hybridaPink Wave / T16

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
1.Gene aadA (encodes for aminoglycoside adenylyltransferase that confers resistance against the antibiotics streptomycin/spectinomycin) originating from Escherichia (E) coli and 2. gene uidA (encodes for beta-D-glucuronidase) E. coli were introduced into petunia chloroplasts under control of a plastid specific 16S rrn promoter

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
aadA coding region from Escherichia coli under control of the plastid specific 16S rrn promoter of Brassica napus coding for an aminoglycoside adenylyltransferase and conferring resistance to the antibiotics streptomycin/spectinomycin, 3’BnpsbC: 3’ regulator element of PsbC-Gens of B. napus as transcription terminator;
uidA coding region from Escherichia coli under control of the plastid specific 16S rrn promoter of Nicotiana tabacum coding for a beta-D-glucuronidase which serves as a reporter gene, 3’NtpsbA: 3’ regulator element of PsbA-Gens of N. tabacum as transcription terminator

6. Brief description of the method used for the genetic modification:
Leaf slices of Petunia hybrida var. Pink Wave were used for plastid transformation with the plastid transformation vector pUM73 by particle bombardment according to Zubko et al. (2004) . The integration of foreign DNA took place by homologous recombination. The plant regeneration was performed on MS medium containing the antibiotics streptomycin and spectinomycin for selection of transformed tissue.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Evaluation of pollen mediated transfer rates of transgenes located in the plastid genome in field grown transgenic petunia (Event T16) with focus on:
-evaluation of paternal inheritance rate of petunia plastids in comparison to tobacco with ~0,1 ‰ probability of occurrence (Svab and Maliga, 2007) .
-evaluation of cross pollination rates after unintended distribution of T16 plastid DNA by pollen
-evaluation of cross pollination rates with non transgenic petunia recipient W115

2. Geographical location of the site:
Thulendorf (Mecklenburg/Vorpommern)

3. Size of the site (m2):
In 2009 we will test two different experimental setups. For the first setup the pollen pressure is high (pollen recipients in each seventh row) and in the second setup the pollen pressure is low (pollen recipients in each second row). If it is not possible to verify statistically the paternal inheritance of transgenes located in the plastid genome due to few or undetectable outcrossing events in 2009, we will double the number of non transgenic recipients and we will only use that experimental setup with a high pollen pressure in the following years.
2009: 477 m2
2010: 477 or 1620 m2
2011: 477 or 1620 m2
2012: 477 or 1620 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no scientific reason to assume that the synthesis of AADA and GUS as new properties of petunia may lead to changes in reproduction, dispersion, persistence or invasiveness of these plants compared to conventional petunia plants. On basis of current experience the transgenic plants do not differ from non-transgenic plants in growth, size, phenotype and seed formation. Under field conditions there is no selective pressure that might lead to a selective advantage of any of the transgenic lines. Due to current experience no effect of the proteins in the plants on pest or beneficial organisms or on health and environment is expected.

Brief description of any measures taken for the management of risks:
Laying and harvesting machinery will be cleaned on site to prevent the dispersal of GM seeds. Harvested plant material will be transported from the site in closed and labelled containers to the laboratories for analyses. The area will be controlled for volunteers for one consecutive year. During the release period the Field Manager and trained personnel will monitor the trial site at defined intervals. After the experiments the transgenic material will be completely removed from the soil.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The field trials are designed to investigate the environmental impacts:
- whether the probability of paternal inheritance measured for tobacco can be transferred to other cultivated plants
- whether the unintended distribution of transgenes by cross-pollination with conventional crops can be reduced due to their localisation in the plastid genome

Literature Cited

Svab Z, Maliga P (2007). Exceptional transmission of plastids and mitochondria from the transplastomic pollen parent and its impact on transgene containment. Proceedings of the National Academy of Sciences of the United States of America 104:7003-7008.
Zubko MK, Zubkot EI, van ZK, Meyer P, Day A (2004). Stable transformation of petunia plastids. Transgenic Res 13:523-530.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
Not known