Notification report

General information

Notification Number

Member State to which the notification was sent
Czech Republic

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Transfer of genes conferring resistance to Plum pox virus

Proposed period of release:
01/11/2017 to 31/12/2027

Name of the Institute(s) or Company(ies)
Crop Research Institute, Prague;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
plumrosaceaeprunusprunus domesticahybrids of GM clone C5

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Clone C5 called in USA "HoneySweet" plum has been prepared by transformation using an Agrobacterium tumefaciens vector carrying the following genes:
- PPV-CP gene (coat protein gene of Plum pox virus) for resistance to Plum pox,
- neomycin phosphotransferase (marker gene conferring resistance to kanamycine),
- beta-glucuronidase (selection marker)
Phenotype: PPV CP gene is not translated. Resistance of the plum is based on RNA interference.

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
PPV-CP gene is a coat protein gene of Plum pox virus,
neomycin phosphotransferase II gene is a selection marker gene conferring resistance to kanamycine; the source is Escherichia coli strain K12,
beta-glucuronidase gene is a selection marker gene

6. Brief description of the method used for the genetic modification:
Hypocotyl slices of plums were transformed using Agrobacterium tumefaciens carrying the vector with the insert.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Plum tree is not a forest tree species.

Experimental Release

1. Purpose of the release:
The purpose of the release of the plum tree hybrids with HoneySweet, that carries the PPV CP gene, is evaluation of their PPV resistance in field conditions and of general agronomic performance including fruit quality.
Environmental safety, e.g. gene flow will be evaluated by the same field trial.

2. Geographical location of the site:
The experimental plot of the Crop Research Institute, located in Prague - Ruzyne, Czech Republic.

3. Size of the site (m2):
900 m2 is the total area of the experimental field. Currently ongoing trial with HoneySweet occupies area approx. 530 m2. The new hybrids will be planted in its vicinity.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The parental GM plum of the newly released hybrids, called HoneySweet, has been tested in a field trial on the same site since 2003. Its potential environmental impact is almost zero, since no case of a gene transfer to nearby growing plums (border rows of the experiment) has been found so far.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Note especially if the introduced traits could directly or indirectly confer an increased selective advantage in natural environments; also explain any significant expected environmental benefits
The only new trait of the genetically modified plums is the PPV resistance, which does not create any effect on their natural environment. Plums generally do not produce much generative progeny, so a potential accidental transfer of pollen to a compatible plant would have minimal probability to create a new plant with the transgene.
So far no risk to human health and the environment has been identified. No immediate or delayed adverse effects as a result of the direct or indirect interaction of the genetically modified plum with the environment are expected.

Brief description of any measures taken for the management of risks:
The field trial is located in an isolated area of the Prague international airport in Prague - Ruzyne. A grass area prevails in a diameter of 1 km from the field trial. No plums flowering in the same time are growing in this area. This unique location creates natural barrier against accidental pollen transmission by pollinating insects.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The new plum hybrids with HoneySweet plum are designed to transfer the resistance against PPV into cultivars with high fruit quality, providing customers in areas with high natural PPV infection pressure with certainty of quality plum fruits. The field trial should evaluate the suitability of the new hybrids for this purpose, assuring also their environmental safety.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
19/12/2017 00:00:00