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Notification report


General information

Notification Number
B/SE/18/01775

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
29/01/2018

Title of the Project
Potato with altered resistance to pathogens

Proposed period of release:
01/05/2018 to 31/12/2018

Name of the Institute(s) or Company(ies)
Swedish University of Agricultural Sciences SLU, Department of Plant Protection Biology, P. O Box 102, 230 53, Alnarp.;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosum

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The trial involves three different transgenic lines, all with a mutated tomato ALS / CSR gene that provides resistance to herbicidal chlorosulfuron (CS), and a Phytophthora infestans gene. In addition to the ALS / CSR gene, the SLJ24895 has the Rpi-amr3i gene from Solanum americanum. SLJ24896-S has the Rpi-amr1e gene from Solanum americanum. SLJ24897 has the Rpi-vnt1.1 gene from Solanum venturii.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
SLJ24895 TDNA; contains from left to right border sequence; left border sequence (LB) originating from Agrobacterium tumefaciens, Acetolactate Synthase / Chlorosulfuron Resistance (ALS / CSR) Promoter, Acetolactate Synthase / Chlorsulfuron Resistance Gene (ALS / CSR), Acetolactate Synthase / Chlorosulfuron (ALS / CSR) Terminator, all from Tomato, Rpi-Amr3i promoter, Rpi-amr3i gene, Rpi-amr3i terminator, all from Solanum americanum, right boundary sequence (RBO).

SLJ24896-S TDNA; contains from left to right border sequence; left border sequence (LB) originating from Agrobacterium tumefaciens, Acetolactate Synthase / Chlorosulfuron Resistance (ALS / CSR) Promoter, Acetolactate Synthase / Chlorsulfuron Resistance Gene (ALS / CSR), Acetolactate Synthase / Chlorosulfuron (ALS / CSR) Terminator, all from Tomato. Rpi Amr1e promoter, Rpi amr1e gene, Rpi amr1e terminator, all from Solanum americanum. Right border sequence (RBO).

SLJ24897 TDNA; contains from left to right border sequence; left border sequence (LB) originating from Agrobacterium tumefaciens, extra left boundary sequence from an octopintype Ti plasmid from Agrobacterium tumefasciens, Acetolactate synthase / chlorsulfuron resistance (ALS / CSR) promoter, acetolactate synthase / chlorsulfuron resistance gene (ALS / CSR), acetolactate synthase / chlorsulfuron (ALS / CSR) terminator, all from tomato. Rpi-vnt1.1 promoter, Rpi-vnt1.1 gene, Rpi-vnt1.1 terminator, all from Solanum venturii. Right boundary sequence (RBO).


6. Brief description of the method used for the genetic modification:
Overexpressions of individual genes have been used. For transformation of potatoes, a binary vector system has been used where genes to be transmitted are found within boundary sequences that form a transfer DNA (T-DNA). The DNA mobilized functions are contained in a modified Ti plasmid that is not transferred to the plant. For the transformation of T-DNA into potatoes, Agrobacterium tumefaciens containing the vector has been used. Hypocotyl tissue has been transformed and transgenic shots have been selected with the herbicide chlorosulfuron (CS). After transformation, Agrobacterium has been killed with Cefotaxim and timentine.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The purpose is to investigate whether potatoes with different genetic modifications are more resistant to Phytophthora infestans as well as to evaluate the agricultural value. We want to compare the different resistance genes under Swedish field conditions that iharbours a varied population of P infestans. We also want to understand how the plants' own resistance mechanisms work. In addition, producing field-material for laboratory trials, and producing tubers for the following year's field trials. Our long-term goal is resistance to potato leaf mold. The experiment is for research purposes only ..

2. Geographical location of the site:
Scania (Kristianstad, Lomma and Kävlinge)

3. Size of the site (m2):
10000 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Potatoes can be propagated via tubers and less so via seeds. Potato tubers are frost sensitive and are therefore dependent on temperature to overwinter in the field. Overwintering of tubers can occur in southern Sweden after a mild winter. Seeds can survive independent of temperature. Very rarely occur waste plants of potatoes in the cultivated landscape since seeds and any tubers are destroyed at the next year's tillage, use of herbicides and competition of the subsequent crop. Potato occurs only in the agricultural landscape. There is no information about potato plants spreading in the wild. Potatoes also have no compatible relatives in Europe and therefore can only cross with other farmed potatoes. The genetic material can be transferred via pollen and crossing to other potatoes in farming areas. The reported dispersion distance for potato pollen is very short and the proposed distance of 20 m to other cultivated potato exceeds spreading distance and is therefore considered sufficient to prevent accidental crossing. The modified potatoes are predicted not to have any new toxic or allergenic substances. Resistance to P. infestans could in theory provide an advantage against non-resistant potato varieties since this disease is very common. If crossing or other type of distribution would occur, the altered resistance can be transferred, but there is no evidence that the potato blight - tolerant potato varieties grown today are more invasive or competitive outside of the cultivated landscape. Increased resistance is assumed not give any change in the viability of the modified potatoes. Altered pathogen resistance is not considered to give an increased risk for health and environment.

Brief description of any measures taken for the management of risks:
In order to minimize the spread of pollen to other farmed potatoes (according to the literature is the distance of the spread of potato pollen maximum of ten meters), a safety distance of 20 m will be held. All flower buds from the genetically modified plants will be removed. Cleaning of machinery, tools, and transportation vehicles will be done after contact with the modified lines. The field will be checked, post-harvest for growth of surviving tubers. The field will not be used to plant potatoes again until at least one growing season without surviving potatoes is observed. Potential waste plants will be documented and eliminated either mechanically or with herbicides. Potatoes will be destroyed either by freezing and composting, autoclaving or by incineration. Transport of harvested potatoes will be done by car or truck in sealed double bags. Transportation will not be done in conjunction with other potatoes. Harvested potatoes will be stored in SLU's premises licensed for such activities. Analyses of the material will be in SLU's premises and leftover crop residues will be destroyed by autoclaving or incineration.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
24/05/2018 00:00:00
Remarks: