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Notification report


General information

Notification Number
B/SE/17/567

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
13/01/2017

Title of the Project
Production of monounsaturated 14 and 16 carbon fatty acids and their incorporation into triacylglycerol and vaxester in Camelina sativa seeds

Proposed period of release:
04/01/2017 to 31/12/2021

Name of the Institute(s) or Company(ies)
Swedish University of Agricultural Sciences, Department of Plant Breeding;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
Gold-of-pleasure, false flaxBrassicaceaeCamelinasativaSuneson

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The events which are applied for has been transformed in two or three steps. The application concerns nine different combinations of modifications in Camelina sativa. In step one and two, marker genes for a flourescent protein and a protein for phosphinothricin tolerance as well as thioesterases with a preference for 14 or 16 carbon chains and desaturases with preference for 14 or 16 carbon chains which results in an increase of corresponding fatty acids in the vegetable oil of the seed.This has resulted in three different modifications: CamiTAG1 containing 2 and 3 under B.4., CamiTAG2 containing 2 and 4 under B.4. and CamiTAG3 containing 1 and 5 under B4.4.

In step three a marker gene for a flourescent protein has been introduced together with genes for a fatty acid reductase and wax synthase which yields wax ester oil in the seed consisting of an esterification between a fatty alcohol and a fatty acid. This has resulted in six different modifications: CamiWE1.1 containing 2, 3 and 6 under B.4., CamiWE1.2 containing 2, 3 and 7 under B.4., CamiWE2.1 containing 2, 4 and 6 under B.4., CamiWE2.2 containing 2, 4 and 7 under B.4., CamiWE3.1 containing 1, 5 and 6 under B.4. and CamiWE3.2 containing 1, 5 and 7 under B.4.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
1. pBin_CpuFATB1: T-DNA fragment, LB, 98 bp, Agrobacterium tumefaciens, border for intended integration; Ubi3 fragment, 117 bp, Solanum, vector residue; glycinin promoter, 702 bp, Glycine max, promoter for seed expression, CpuFATB1, 1251 bp, Cuphea avigera var. pulcherrima, facilitate export of fatty acids from plastid, C16:0, glycinin terminator, 458 bp, Glycine max, transcriptional termination; CsVMV promoter, 528 bp, Cassava vein mosaic virus, promoter for general expression, DsRED, 684 bp, Discosoma sp., coding for fluorescent protein as visual marker, T-DNA fragment, Nos ter, RB, 370 bp, Agrobacterium tumefaciens, transcriptional termination and border for intended integration

2. pBin_CpFATB2: T-DNA fragment, LB, 98 bp, Agrobacterium tumefaciens, border for intended integration; Ubi3 fragment, 117 bp, Solanum, vector residue; glycinin promoter, 702 bp, Glycine max, promoter for seed expression, CpFATB2, 1236 bp, Cuphea palustris, facilitate export of fatty acids from plastid, C14:0, glycinin terminator, 458 bp, Glycine max, transcriptional termination; CsVMV promoter, 528 bp, Cassava vein mosaic virus, promoter for general expression, DsRED, 684 bp, Discosoma sp., coding for fluorescent protein as visual marker, T-DNA fragment, Nos ter, RB, 370 bp Agrobacterium tumefaciens, transcriptional termination and border for intended integration

3. pBin_CpaE11: T-DNA fragment, LB, 98 bp , Agrobacterium tumefaciens border for intended integration; T-DNA fragment, Nos ter, 260 bp, Agrobacterium tumefaciens, transcriptional termination; BAR gen, 552 bp, synthetic ex. Xanthomonas sp., herbicid tolerance to BASTA; T-DNA fragment, Nos prom., 608 bp, Agrobacterium tumefaciens, promoter for general expression; glycinin promoter, 702 bp, Glycine max, promoter for seed expression, CpaE11, 1236 bp, Choristoneura parallela, fatty acid desaturation, C14:0, glycinin terminator, 458 bp, Glycine max, transcriptional termination; DsRED trunkerad, 349 bp, Discosoma sp., non-functional, coding for fluorescent protein; T-DNA fragment, Nos ter, RB, 370 bp, Agrobacterium tumefaciens, transcriptional termination and border for intended integration

4. pBin_AveD11: T-DNA fragment, LB, 98 bp , Agrobacterium tumefaciens, border for intended integration; T-DNA fragment, Nos ter, 260 bp, Agrobacterium tumefaciens, transcriptional termination; BAR gen, 552 bp, synthetic ex. Xanthomonas sp., herbicid tolerance to BASTA; T-DNA fragment, Nos prom., 608 bp, Agrobacterium tumefaciens, promoter for general expression; glycinin promotor, 702 bp, Glycine max, promoter for seed expression, AveD11, 990 bp, Argyrotaenia velutinana, fatty acid desaturation, C14:0, glycinin terminator, 458 bp, Glycine max, transcriptional termination; DsRED trunkerad, 349 bp, Discosoma sp., non-functional, coding for fluorescent protein; T-DNA fragment, Nos ter, RB, 370 bp, Agrobacterium tumefaciens, transcriptional termination and border for intended integration

5. pBin_AtrD11: T-DNA fragment, LB, 98 bp , Agrobacterium tumefaciens, border for intended integration; T-DNA fragment, Nos ter, 260 bp, Agrobacterium tumefaciens, transcriptional termination; BAR gen, 552 bp, synthetic ex. Xanthomonas sp., herbicid tolerance to BASTA; T-DNA fragment, Nos prom., 608 bp, Agrobacterium tumefaciens, promoter for general expression; glycinin promotor, 702 bp, Glycine max, promoter for seed expression, AtrD11, 981 bp, Amyelois transitella, fatty acid desaturation, C16:0, glycinin terminator, 458 bp, Glycine max, transcriptional termination; DsRED trunkerad, 349 bp, Discosoma sp., non-functional, coding for fluorescent protein; T-DNA fragment, Nos ter, RB, 370 bp, Agrobacterium tumefaciens, transcriptional termination and border for intended integration

6. pXZP_HarFAR_SaWS: T-DNA fragment, RB, 163 bp, Agrobacterium tumefaciens, border for intended integration; napin promotor, 356 bp, Brassica napus, promoter for seed expression, HarFAR, 1365 bp, synthetic Helicoverpa armigera, fatty acid reductase, T-DNA fragment, Ocs ter, 708 bp, Agrobacterium tumefaciens, transcriptional termination; napin promotor, 356 bp, Brassica napus, promoter for seed expression, SaWS, 1105 bp, Sorbus aucuparia, wax synthase, T-DNA fragment, Ocs ter, 708 bp, Agrobacterium tumefaciens, transcriptional termination; napin promotor, 356 bp, Brassica napus, promoter for seed expression, catalase intron, 196 bp, Ricinus communis, intron for plant specific function, beta-conglycinin signal, 90 bp, Glycine max, peptide signal for secretion, GFP N-terminal, 399 bp, synthetic Aequorea victoria, coding for fluorescent protein as visual marker, catalase intron, 190 bp, Ricinus communis, intron for plant specific function, GFP C-terminal, 333 bp, syntetisk Aequorea victoria, coding for fluorescent protein as visual marker, T-DNA fragment, Nos ter, 253 bp, Agrobacterium tumefaciens, transcriptional termination, T-DNA fragment, LB, 785 bp, Agrobacterium tumefaciens, border for intended integration

7. pXZP_HarFAR_MhWS: T-DNA fragment, RB, 163 bp, Agrobacterium tumefaciens, border for intended integration; napin promotor, 356 bp, Brassica napus, promoter for seed expression, HarFAR, 1365 bp, synthetic Helicoverpa armigera, fatty acid reductase, T-DNA fragment, Ocs ter, 708 bp, Agrobacterium tumefaciens, transcriptional termination; napin promotor, 356 bp, Brassica napus, promoter for seed expression, MhWS, 1422 bp, synthetic Marinobacter hydrocarbonoclasticus, wax synthase, T-DNA fragment, Ocs ter, 708 bp, Agrobacterium tumefaciens, transcriptional termination; napin promotor, 356 bp, Brassica napus, promoter for seed expression, catalase intron, 196 bp, Ricinus communis, intron for plant specific function, beta-conglycinin signal, 90 bp, Glycine max, peptide signal for secretion, GFP N-terminal, 399 bp, synthetic Aequorea victoria, coding for fluorescent protein as visual marker, catalase intron, 190 bp, Ricinus communis, intron for plant specific function, GFP C-terminal, 333 bp, synthetic Aequorea victoria, coding for fluorescent protein as visual marker, T-DNA fragment, Nos ter, 253 bp, Agrobacterium tumefaciens, transcriptional termination, T-DNA fragment, LB, 785 bp, Agrobacterium tumefaciens, border for intended integration


6. Brief description of the method used for the genetic modification:
Camelina sativa of the cultivar Suneson has been transformed using Agrobacterium tumefaciens transformation of inflorescence, i.e. floral dip. Inflorescence is in this method dipped in an Agrobacterium solution where the bacterium contains the gene construct which is of interest to transfer to the plant chromosome. Produced seeds are subsequently investigated regarding gene transfer, usually using a visual marker as DsRed, germination of seeds on a selective medium or treatment of next generation plants with a herbicide that the event has received a tolerance for by transformation.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
not applicable

Experimental Release

1. Purpose of the release:
To investigate stability of oil composition under field conditions (triacylglycerol and wax ester) and generating a sufficient volume of seeds for extraction and technical processing of the oil to be used for functional testing of derivates of the same.

2. Geographical location of the site:
Lomma municipality, Kävlinge municipality, Halmstad municipality, Kristianstad municipality

3. Size of the site (m2):
up to 30,000

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Camelina sativa modified according to the first two steps described under B.2. has been field tested in Nebraska, USA during 2016. These have then not shown any impact on the environment or human health beyond conventional Camelina sativa. One modification resulted in reduced seed quality.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
There is no basis for an assumption that the added properties would yield a selective advantage in natural environments.

Brief description of any measures taken for the management of risks:
All machines that are used for the field trial are thoroughly cleaned before they can leave the area of the designated field trial. A buffer area with a cereal is sown around the transgenic lines followed by another buffer area of conventional Camelina sativa which then provides the boundary of the designated trial site. The trial site is inspected twice a week. A zone outside the designated trial site is inspected once per month to note and remove any sexually compatible Camelina relatives. At harvest, threshed material is collected in labelled double bags. Straw and non harvested material is left on the designated trial site and thereafter the trial site is burnt. The trial site is inspected two months after harvest to note and remove any volunteers. The designated trial site is in fallow the following year and is inspected April to September for noting and removal of any volunteers. A zone beyond the designated trial site is inspected once during the growing season for noting and removal of any Camelina sativa including sexually compatible relatives. This inspection is repeated for a total of three years after the trial or two if no volumteers are found year one and two. If volunteers are noted year three another cycle of inspection is started.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Documentation will be performed regarding observations during growing season and inspections of designated trial site with surrounding inspected zone.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Not known