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Notification report


General information

Notification Number
B/SE/17/19386

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
12/12/2017

Title of the Project
Aspen as a model system for wood quality - Prolongation

Proposed period of release:
01/03/2018 to 01/10/2019

Name of the Institute(s) or Company(ies)
Sveriges Lantbruksuniversitet, Umeå Plant Science Centre, Skoglig genetik och växtfysiolog;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/SE/11/3992;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-T89

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application includes lines with 13 different constructs, which have been selected since they induce increased growth or better saccharification. The genes either alter xylan structure, or decrease lignin content, or decrease pectin content, or change the polysaccharide metabolism such that the wood chemistry is positively affected (increased saccharification potential, improved mechanical properties).

The inserted marker genes are coming from 4 vectors, described in detail in B4, and the transferred sequences from these vectors include neomycin phosphotransferase (Kanamycin resistance), promotors of nopaline synthase, 35S, or WP, and terminator 35S. In one case the reporter genes encoding eGFP and beta glucuronidase (GUS) are used to monitor the expression stability and as a transgenic control.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
This application consists of 13 different gene constucts where 11 Populus genes were up- or down regulated, and two fungal genes have been expressed. All these gens were selected based on their enzymatic activities that target chemistry of cell walls, or alternatively based on the effects of these genes on wood chemistry as recorded in greenhouse experiments. Four different vectors are used 1) pK7GWIWG2(I), 2) pK2GW7, 3) pK2GW7-WP and 4) pKGWFS7.

The Genes that were introduced from these vectors are: 1) 35S promotor (p35S) followed by a gene of interest in two inverted copies with an intron in between, followed by 35S terminator (t35S), neomycin phosphotransferase encoding gene (NPTII) for kanamycin resistance driven by the nopaline synthase promotor (pNos), 2) p35S promotor followed by gene of interest either in sense orientation or in two inverted copies with an intron in between them, and t35S terminator, NPTII driven by pNos promotor, 3), wood-specific promotor (WP) followed by the gene of interrest either in sense orientation or in two inverted copies with intron in between them, followed by t35S terminaror, and NPTII gene driven by pNos promotor, 4) WP promotor followed by the reporter eGFP-GUS genes and t35S terminator, NPTII gene driven by pNos promotor.

The 11 Populus genes code for 1) a cinnamate 4-hydroxylase (C4H) involved in lignin biosynthesis, 2) a laccase involved in lignin biosynthesis, 3) an aspartate protease involved in xylan biosynthesis 4) a glycosyl transferase family GT8, PARVUS gene ortologue, involved in xylan biosynthesis 5) a pectate lyase, 6) two glycosyl transferases from family GT43 involved in xylan biosynthesis, 7) four REDUCED WALL ACETYLATION family proteins involved in xylan acetylation. Two fungal genes encode two different xylan acetyl esterases. In addition, one control construct is expressing only eGFP-GUS reporter genes driven by the WP promotor.


6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind-pollinated and with wind-dispersed seeds, and can also propapagate from suckers.

Experimental Release

1. Purpose of the release:
The aim is to see if any of the constructs shows improved wood quality in the field conditions, as was observed in the greenhouse. In addition, we will investigate if plant growth and herbivore- or pathogen-interactions are modified. The particular clone (T89) is not useful for commercial forestry in Sweden, so none of these lines are aimed at being commercialized. However, if some genes give increased growth without any penalty, they may later be used in other genetic background.

2. Geographical location of the site:
Våxtorps plantskola, Laholms kommun

3. Size of the site (m2):
25 000 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
This is a continuation of an ongoing experiment, established in 2014. No potential environmental or human health impact has been detected.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
We hope that some of the lines will show good growth in the field and enhenced wood quality traits .If so, this may lead to better-yielding tree varieties for fibre- or energy production. It is expected that the traits would not give any particular advantave in natural environment, it is most likely that these lines will need to be arificially maintained, as majority of genetically improved crops.

Brief description of any measures taken for the management of risks:
The trees will not be allowed to flower. Regular inspection for early detection of possible flowering buds (those lines will be immediately removed). Fence and buffer zone to surrounding forests and regular removal of suckers will prevent spreading by vegetative propogules.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
18/04/2018 00:00:00
Remarks: