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Notification report


General information

Notification Number
B/SE/16/713

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
26/01/2016

Title of the Project
Development of Lepidium campestre as a new and cover oil crop for better health and environment

Proposed period of release:
01/05/2016 to 31/12/2020

Name of the Institute(s) or Company(ies)
Department of Plant biology
Swedish University of Agricultural Sciences, Uppsala;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
Field cressBrassicaceaeLepidiumcampestreacc. NO94-7

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Three transgenic lines will be included in the field trial: EL18-1, EL-HO, EL-PS.

1. Line EL18-1 shows a significant increase in the oleic acid content in its seed oil. GC analysis of the seed oil has shown that the average oleic acid content has increased to over 70% compared with 11% in the wild type in the third generation. We would like to make more field trials for further evaluation of this line.
The line contains also the nptII gene, which has previously been proved not to affect the plant growth and development.

2. Line EL-HO shows increased oil content with 20% in average compared with the wild type from 3 generations in biotron. We would like to evaluate the line under field conditions.
The line contains also the nptII genes.

3. Line EL-PS shows clearly increased pod shatter resistance, tested with a shake machine. We need to perform the field trial to confirm how the line will behave under field conditions.
The line contains also the nptII gene.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
1. LcFAE1-RNAi and LcFAD2-RNAi construct is made using a fragment of FAE1 and FAD2 gene, respectively, of Lepidium campestre. The FAE1 gene is coding for the enzyme fatty acid elongase that increase the carbon chain length from C18 to C20 or above. The FAD2 gene is coding for the enzyme fatty acid desaturase that desaturates oleic acid to linoleic acid. The intron comes from Flaveria trinerva and Ricinus communis in order to down regulate the FAE1 and FAD 2 gene, and thereby the oleic acid content.

2. WRI1 gene comes from Arabidopsis thaliana in order to increase the seed oil content.

3. LcIND-RNAi construct is made using a gene fragment of the IND gene of Lepidium campestre. The IND gene is involved in pod shatter control. The intron comes from Flaveria trinerva and Ricnus communis in order to down regulate the IND gene, and thereby reduce the pod shatter.

nptII gene comes from Escherichia coli in order to select transformants, but it has no effects on plant growth and developmen


6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated gene transfer was used for the genetic modification

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The main aim of the field trial is to evaluate the performance of the GM lepidium lines under field conditions regarding the oil composition, especially the oleic acid content, oil content and pod shatter resistance. We also intend to observe the tolerance or resistance of the GM lines to bio- and abiotic stresses, such as insect or disease resistance. The Release will also allow us to multiply the seed material from the three lines to be used in future field trials.

2. Geographical location of the site:
The release will be in collaboration with Hushållningssällskapet in one of the commmunities: Lomma kommun, Kävlinge kommun and Kristianstad kommun.

3. Size of the site (m2):
The total area of release in one year during 2016-2020 will not exceed 5000 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The modified or introduced trait in the three GM-lepidium lines are only concerning an alteration in the composition of the seed oil, seed oil content and pod shatter. In the EL18-1 line, the level of the existing oleic acid has been increased from 11% to over 70%. The EL-HO line has increased oil content with average of 20%. In the EL-PS line, the pod shatter resistance has been increased.
The increased level of oleic acid and seed oil content should not give any selective advantages in a natural envirionment. The increased pod shatter resistance should reduce the risk of seed dipersing in nature.


Brief description of any measures taken for the management of risks:
The trial fields with the GM-lepidium lines will be surrounded by a 10 meters wide border of a barrier crop (barley or wild type lepidium) that is big enough for mashineries to turn around and to clean out the harvesting equipment.
After harvesting the seeds from the GM-lepidium lines, the trial field will be burned to destroy any remaining crop (including barrier crop). Additional straw will be added onto the field to facilitate the burning if needed.
Measures are made so to limit the buildup of a seed bank by a light harrow after the harvest and the burning of the field that will stimulate GM-lepidium seeds dropped to the ground to germinate. Once the trials are finished, the field will lay in fallow and during this time it will be harrowed regularly over the year to facilitate seeds to grow and seedlings to be controlled.
Beside the dates for recording the crops development, the lepidium field will be monitored twice a month in the growing season.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
07/07/2016 00:00:00
Remarks: