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Notification report


General information

Notification Number
B/SE/16/3494

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
27/04/2016

Title of the Project
Aspen as model system

Proposed period of release:
01/08/2016 to 01/08/2021

Name of the Institute(s) or Company(ies)
UmeƄ University, Department of Plant Physiology;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-T89 and 864012
poplarsalicaceaepopuluspopulus deltoides

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application consists of different gene constucts where Populus genes have been down- or upregulated, two where a gene from Arabidopsis is expressed and one where a deletion in an aspen gene has been introduced using CRISPR. They have been selected since we beleve that that have changed phenlogy. In addition we apply for a line where a CAS9 gene is expressed. Five different vectors are used 1) pGREENII 2) pK7GWIWG2(I), 3) pK2GW7, 4) pGWB502 and p) pEARLY201. In overexpressor, the genes are driven by either the CaMv 35S promoter (or in two cases aspen promoters), the RNAi constructs consist of the gene fragment in two inverted copies, with an intron, driven by the 35S promoter. Genes introduced are 1) CAS9 driven by the 35S promoter (from Cauli flower mosaic virus) and a NPTII gene 2) the same plus sgRNAs directed against the FT1 gene, 3) a RNAi construct with fragments of the SVP gene from Populus in two inverted copies with an intron in between, driven by the 35S promoter and followed by a terminator, and neomycin phosphotransferase NPT-II driven by the Nos-promotorn 4) the SVP gene driven by the 35S promoter and NPTII as above, 5) as 3 but with the PhyB gene, 6) the same as 4 but with the CO1 gene, 7) the same as 4 but with the CO2 gene, 8) the same as 4 but with the GI gene, 9) the same as 4 but with the GIL gene, 10) the same as 3 but with the LFY gene, 11) the same as 4 but with the RCAR gene 12) the same as 3 but with the RCAR gene, 13) a miRNA construct reducing the expresison of the OPR3 gene, and the hygromycin resistance gene 14) the same as 13, but also with an RNAi constuct (like in 3) for the FT1 gene 15) the same as 13 but with a miRNA-construct for the AOS gene 16) the same as 14 but with a miRNA construct for the AOS gene, 17) the GA20ox gene driven by the MEE1 promoter (from Populus) 18) same as 17 but with the MEE2 promoter (from Populus), 19) the same as 4 but with the FT1 gene (and instead a Km-resistance gene) and 20) the same as 19 but with the FT2 gene. The CAS9 gene in 1) comes orginally from bacteria, although the codon usage has been changed for better expression in plants, and the sgRNA in 2) is synthetic. The Populus genes in 3-16 and 19-20 - and the Arabidopsis gene in 17 and 18 - were selected since they were expected to be regulate the phenology of the tree, this was also proven in experiments under controlled conditions. The Arabidopsis gene has the same enzymatic function as a Populus gene. Some constructs have been introduced into the T98 background, some in 864012, but as the risk evaluation will be the same the application is for all constructs in both hybrid aspen lines, plus natural aspen genotypes.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
See B2. In addition to the functions of the genes in the vectors - to allow transformation and selection - have the genes been selected to influence the phenology of the tree.

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind-pollinated and with wind-dispersed seeds, and can also propapagate from suckers. To our knowledge, the particular clone has never flowered in Sweden during at least 20 years of growth. Aspen does not normally flower earlier than after 20 years.

Experimental Release

1. Purpose of the release:
Basic research on tree phenology

2. Geographical location of the site:
Våxtorps plantskola, Laholms kommun

3. Size of the site (m2):
Less than a hectar

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Probably not, optimal phenology is probably already selected by nature

Brief description of any measures taken for the management of risks:
Regular inspection for early detection of possible flowering (those would be removed). Fence and buffer zone to surrounding forests. Removal of suckers.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
23/08/2016 00:00:00
Remarks: