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Notification report


General information

Notification Number
B/SE/16/10775

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
12/02/2016

Title of the Project
Potato with altered resistance to pathogens

Proposed period of release:
03/01/2017 to 31/12/2021

Name of the Institute(s) or Company(ies)
Swedish University of Agricultural Sciences SLU, Department of Plant Protection Biology, P. O Box 102, 230 53, Alnarp.;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumDesiree, King Edward, Kuras

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The trial comprises five different transgenic plants with: a) an over-expressed resistance gene (Rpi-blb1) involved in Phytophthora infestans resistance, neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker. B) three over-expressed resistance genes (Rpi-vnt1.1, Rpi-blb1, Rpi-blb2) involved in Phytophthora infestans resistance, neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker. C) an over-expressed resistance gene (Dulc1) involved in resistance, neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker. D) an over-expressed resistance gene (35S-Dulc1) involved in resistance, neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker. E) down-regulated suseptibility gene (dmr6), with neomycin phosphotransferase II (nptII) coding for kanamycin resistance as a marker.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The application includes five different transformed plants:
Blb1 - left border sequence (LB) from Agrobacterium tumefaciens, nopaline synthase promoter (Pnos) from Agrobacterium tumefaciens, neomycin phosphotransferase II coding sequence (nptII) from Escherichia coli (marker), nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, Rpi-blb1 promoter- resistance gene and terminator from Solanum bulbocastanum, right border (RB) from Agrobacterium tumefaciens.

3R - left border sequence (LB) from Agrobacterium tumefaciens, 35S terminator (35S-T) from cauliflower mosaic virus (CaMV), neomycin phosphotransferase II coding sequence (nptII) from Escherichia coli (marker), 35S promoter (P35S) from cauliflower mosaic virus (CaMV), Rpi-vnt1.1 promoter- resistance gene and terminator from Solanum venturii, Rpi-blb1 promoter- resistance gene and terminator from Solanum bulbocastanum, Rpi-blb2 promoter- resistance gene and terminator from Solanum bulbocastanum, right border (RB) from Agrobacterium tumefaciens.

Dlc1 - right border (RB) from Agrobacterium tumefaciens, Dlc1 promoter- resistance gene and terminator from Solanum dulcamara, nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, neomycin phosphotransferase II coding sequence (nptII) from Escherichia coli (marker), nopaline synthase promoter sequence from Agrobacterium tumefaciens, left border sequence (LB) from Agrobacterium tumefaciens.

35S- Dlc1 - left border sequence (LB), nopaline synthase polyadenylation sequence (nosT) from Agrobacterium tumefaciens, neomycin phosphotransferase II coding sequence (nptII) from Escherichia coli (marker), nopaline synthase promoter (Pnos) from Agrobacterium tumefaciens, 35S promoter (P35S) from cauliflower mosaic virus (CaMV), Dlc1 gene from Solanum dulcamara, 35S terminator (35S-T) from cauliflower mosaic virus (CaMV), right border (RB) from Agrobacterium tumefaciens.

Dmr6-RNAi - right border (RB) of Agrobacterium tumefaciens, 35S promoter (P35S) from cauliflower mosaic virus (CaMV), duplicated and inverted fragment of the dmr6 suseptibility-gene from Solanum tuberosum, between the duplication is an intron from pyruvate orthofosfat dikinas (PDK), octopine synthase (ocsT) polyadenylation sequence of Agrobacterium tumefaciens, nopaline synthase promoter (Pnos) of Agrobacterium tumefaciens, neomycin phosphotransferase II coding sequence (nptII) from Escherichia coli, nopaline synthase (nosT) polyadenylation sequence from Agrobacterium tumefaciens and left border sequence (LB) from Agrobacterium tumefaciens.


6. Brief description of the method used for the genetic modification:
Individual genes have been over-expressed or down-regulated. For downregulation RNA interference (RNAi), a naturally occurring system of regulation of genes in cells, was used. For transformation of potato a binary vector system where genes to be transferred can be found inside the border sequences that form a transfer DNA (T-DNA) was used. The DNA mobilization features are available in a modified Ti plasmid that is not transferred to the plant. For transformation of T-DNA to the potato, Agrobacterium tumefaciens containing the vector has been used. Cut potato leaf tissue was transformed and transgenic shoots were selected on antibiotics. After transformation, the Agrobacterium killed with 400 mg/ml Cefotaxime.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
Under field conditions evaluate agricultural value including resistance properties. Produce seed for next year's field trials. Study the stability of the modified features, identify any morphological abnormalities, producing field-grown material for laboratory testing, and produce seed for next year's field trials. The long-term goal is late blight resistance and to gain knowledge about resistance mechanisms. The experiment is only out for research purposes.

2. Geographical location of the site:
Scania (Kristianstad, Lomma and Kävlinge)

3. Size of the site (m2):
<1500 m2 per site. Total: max 4500m2 per year

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Potatoes can be propagated via tubers and via seeds. Potato tubers are frost sensitive and is therefore dependent on temperature to overwinter in the field. Overwintering of tubers can occur in southern Sweden after a mild winter. Seeds can survive independent of temperature. Very rarely occur waste plants of potatoes in the cultivated landscape since seeds and any tubers are destroyed at the next year's tillage, use of herbicides and competition of the subsequent crop. Potato occurs only in the agricultural landscape. There is no information about potato plants spreading in the wild. Potatoes also have no compatible relatives in Europe and therefore can only cross with other farmed potatoes. The genetic material can be transferred via pollen and crossing to other potatoes in farming areas. The reported dispersion distance for potato pollen is very short and the proposed distance of 20 m to other cultivated potato exceeds spreading distance and is therefore considered sufficient to prevent accidental crossing. The modified potatoes are predicted not to have any new toxic or allergenic substances. Resistance to P. Infestans could in theory provide an advantage against non-resistant potato varieties since this disease is very common. If crossing or other type of distribution would occur, the altered resistance can be transferred, but there is no evidence that the potato blight - tolerant potato varieties grown today are more invasive or competitive outside of the cultivated landscape. Increased resistance is assumed not give any change in the viability of the modified potatoes. Altered pathogen resistance is not considered to give an increased risk for health and environment.

Brief description of any measures taken for the management of risks:
In order to minimize the spread of pollen to other farmed potatoes (according to the literature is the distance of the spread of potato pollen maximum of ten meters; OECD, 1997), a safety distance of 20 m will be held. All flower buds from the genetically modified plants will be removed, or alternatively a distance to registered positions for hives of about 3 km will be held. Cleaning of machinery, tools, and transportation vehicles will be done after contact with the modified lines. The field will be checked, post-harvest for growth of surviving tubers. The field will not be used to plant potatoes again until at least one growing season without surviving potatoes is observed. Potential waste plants will be documented and eliminated either mechanically or with herbicides. Plant residues from field cultivation and harvest will be destroyed either by freezing and composting, evaporation, autoclaving or by incineration. Transport of harvested potatoes will be done by car or truck in sealed double bags. Transportation will not be done in conjunction with other potatoes. Harvested potatoes will be stored in SLU's premises. Analyses of the material will be in SLU's premises authorized for such activityand leftover crop residues will be destroyed by autoclaving or incineration..

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known