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Notification report


General information

Notification Number
B/SE/14/6846

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
21/08/2014

Title of the Project
Fast-growing hybrid aspens: what is the relation between growth in greenhouse and in the field? - Prolongation

Proposed period of release:
01/01/2015 to 31/12/2019

Name of the Institute(s) or Company(ies)
Umeå University, Department of Plant Physiology;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/SE/09/12395;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application consists of 15 different gene constucts where Populus genes have been down- or upregulated. They have been selected since they result in increased growth in greenhouse experiments.

Three different vectors are used 1) pK7GWIWG2(I), 2) pK2GW7 and 3) pPCV812-LMX5-Gateway. Genes introduced from these are
1) p35S followed by gene of interest in two inverted copies with an intron in between, followed by a terminator T35S, neomycin phosphotransferase NPT-II driven by the Nos-promotorn
2) p35S followed by gene of interest and a terminator T35S, NPTII driven by Nos-promotor,
3) LMX5 promotorn followed by gene of interest and terminator from nopaline syntase (pANos), Hygromycin resistance gene driven by nopaline syntase promotor (pNos) and bla (β-lactamase) with replicon from pBR322.

The 15 Populus genes codes for 1) an LRR protein 2) a GRAS transcription factor, 3) a phosphatidyl serine synthase (PSS) 4) a WRKY transcription factor 5) a KNOTTED-like homeobox transcription factor 6, 7, 8 och 9) different WRKY transcription factors 10) a bHLH transciption factor 11) praboably a regulator of the gibberelin response 12) a WRKY transcription factor 13) a HD–GLABRA2 transkription factor 14) a SET-domain protein and 15) a WRKT transcription factor.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
See B2. In addition to the functions of the genes in the vectors - to allow transformation and selection - have the 15 genes been selected from a large material there ca 1000 Populus genes have been up- or downregulated. These 15 genes gave increased growth under greenhouse conditions, and we would like to see how they perform in the field. We cannot say that any of the genes has an "intended function" as the selection of the 1000 genes were not based on a known function, just since they had either an interesting expression pattern or was an transcription factor

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind-pollinated and with wind-dispersed seeds, and can also propapagate from suckers. To our knowledge, the particular clone has never flowered in Sweden during at least 20 years of growth

Experimental Release

1. Purpose of the release:
The aim is to see which of the 15 lines, that all show increased growth in the greenhouse, also grow better in the field. In addition, we will investiga if herbivore- or pathogen-interactions are modified. The particual clone (T89) is not useful for commerciall forestry in Sweden, so none of these lines are ained at being commercialized. However, if some genes give increased growth without any penalty, they may later be used in other lines.

2. Geographical location of the site:
Arlösa gård, Halmstad municipality and Våxtorps plantskola, Laholms municipality

3. Size of the site (m2):
Less than a hectar at each site

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
This is a continuation of an ongoing experiment, established in 2010. No potential environmental or human health impact has been detected.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
We hope that some of the lines will show good growth in the field .If so, this may lead to better-yielding tree varieties for fibre- or energy production.

Brief description of any measures taken for the management of risks:
Regular inspection for early detection of possible flowering (those would be removed). Fence and buffer zone to surrounding forests. Removal of suckers.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known