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Notification report


General information

Notification Number
B/SE/12/484

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
13/01/2012

Title of the Project
Increased nitrogen use efficiency in barley

Proposed period of release:
01/05/2012 to 01/05/2012

Name of the Institute(s) or Company(ies)
Swedish University of Agricultural Sciences (SLU), Department of Forest Genetics and Plant Physiology;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
barleypoaceaehordeumhordeum vulgarevulgareGolden Promise

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Two genes encoding the amino acid transporters; lysine histidine transporter (LHT1), amino acid permease 5 (AAP5) has been inserted in barley to modify the uptake of amino acids carried by these amino acid transporters. The genes originate in Arabidopsis thaliana. In addition antibiotic resistant genes has been transformed into these plants to facilitate selection of the transgene plants. The antibiotic resistance transfered has been isolated from different bacterias; e g neomycinfosfotransferas (kanamycin resistens) and hygromycinfosfotransferas (hygromycin resistens).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Three constructs has been used. The vector pBract214 has been used for two different construct and the vector pKLM24GW,3 has been used for one construct
1) pBract214 with AtLHT1, Lysine Histidine Transporter 1 (AT5G40780.1). AtLHT1 from Arabidopsis thaliana is used to increase uptake of acid and neutral amino acids.
2) pBract214 with AtAAP5 Amino Acid Permease 5, (AT1G44100.1). AtAAP5 from Arabidopsis thaliana is used to increase uptake of bacis amino acids.
35S-Hyg-nos has been used in both constructs above. It originates from Cauliflower mosaic virus, the 35S promotor is used to get constant expression of AtLHT1 och AtAAP5. Hyg gives hygromysin resistens. Nos is a terminator.
3) pKLM24GW,3 with the btg26 promotor from Brassica napus and AlaAT, Alanine Aminotransferase from Hordeum vulgare. AlaAT is used to increase the amount of alanine aminotransferase, to increase the nitrogen use efficiency. The btg26 promoter is a root specific promoter that is used to overexpress AlaAT only in the roots.


6. Brief description of the method used for the genetic modification:
Agrobaterium-mediated gene transfer was used for the genetic modification.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The long term aim is to develop barley that have an increased nitrogen use efficiency, e g. that gives the same harvest at lower nitrogen applicaition than the conventional agriculture are using today, to decrease nitrogen leaching to surrounding environments.
The objective is to take the transformed barley lines out in the field is to evaluate the stability of nitrogen use efficiency and gene expression levels. Barley lines overexpressing the Arabidopsis thaliana genes encoding the amino acid transporters responisble for the root uptake of different amino acids (AtLHT1 eller AtAAP5) will be tested and compared to a control (non-modified barley). Overexpression of the amino acid transporter LHT1 in Arabidopsis resulted in significant increase in uptake rate of amino acids in roots and an increased biomass growth on amino acids. We would like to measure the growth and yield of different barley lines with increased amino acid uptake under different fertilization regimes of organic and inorganic nitrogen fertilizers. The trials will also give multiplication of seed material for the proceeding field cultivations and application tests.


2. Geographical location of the site:
During 2012 the release will be at Helgegården in Skepparslöv outside Kristianstad. In 2013-2016 other sites may be added.

3. Size of the site (m2):
The total area of release in one year during 2013-2016 will not exceed 10 000 m2. During 2012 tha plan is to grow the barley in 2 l pots with a 10 meter security border of spring barley. During the following years barley will be cultivated in experimental field plots (120 m2 per barley line) with a 10 meter security border of spring barley.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Genetically modified barley has not been released before in Sweden. Genetically modified barley has been released in other EU countries.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The aim with the modifications is to increase the plant’s ability to use amino acids as a nitrogen source which could influence the cultivated population. Soil organisms might therefore be influenced by this during the growing season. The purpose with this trial is to decrease the nitrification and thereby decrease the leaching of nitrate to surrounding environments.

No increased risks for health and environment are appraised by the inserted traits (genes) since they are of plant origin and the function is the same as in the GM-barley plants.


Brief description of any measures taken for the management of risks:
The trial fields with the GM-barley lines will be sourrounded by a 10 meters wide border of a barrier crop (barley) that is big enough for mashineries to turn around and to clean out the harvesting equipment.
After harvesting the seeds from the GM-barley lines, the trial field will be burned to destroy any remaining crop (including barrier crop). Additional straw will be added onto the field to facilitate the burning.
Measures are made so to limit the buildup of a seed bank by a light harrow after the harvest and the burning of the field that will stimulate GM-barley seeds dropped to the ground to germinate. The year after the field will lay in fallow and during this time it will be harrowed with regular intervals over the year to facilitate seeds to grow and seedlings to be controlled.
It can be added that growing barley plants can not survive a normal winter in the South of Sweden.
Beside the dates for recording the crops development, the barley field will be monitored at least twice a month.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The trial fields with the GM-barley lines will be sourrounded by a 10 meters wide border of a barrier crop (barley) that is big enough for mashineries to turn around and to clean out the harvesting equipment.
After harvesting the seeds from the GM-barley lines, the trial field will be burned to destroy any remaining crop (including barrier crop). Additional straw will be added onto the field to facilitate the burning.
Measures are made so to limit the buildup of a seed bank by a light harrow after the harvest and the burning of the field that will stimulate GM-barley seeds dropped to the ground to germinate. The year after the field will lay in fallow and during this time it will be harrowed with regular intervals over the year to facilitate seeds to grow and seedlings to be controlled.
It can be added that growing barley plants can not survive a normal winter in the South of Sweden.
Beside the dates for recording the crops development, the barley field will be monitored at least twice a month.


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Not known