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Notification report


General information

Notification Number
B/SE/12/2015

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
15/02/2012

Title of the Project
Arabidsopsis as model system

Proposed period of release:
01/06/2012 to 31/12/2016

Name of the Institute(s) or Company(ies)
Umeå University, Department of Plant Physiology;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
thale cressbrassicaceaearabidopsisarabidopsis thaliana

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
T-DNA or transposon KO-mutations of the different Arabdidopsis genes, with lines from the different KO-collections availabe to the scientific community, i e the so-called SALK, Wisman, SAIL, RIKEN. SLAT, GABI-KAT, FLAG and AKF-collections. Different vectors for the different T-DNA KO collections are pROK2, pGV3850 HPT::pkEn2, pCSA110, pDAP101, pAC106, pAC161, pGABI1, pADIS1, pGKB5 and pD991-AP3. Transposon KO-mutations are induced by transposition of existing elements, not introduction of new DNA through transformation. Selectable markers for the different collections are either kanamycin, hygromycin, BASTA or sulfadiazine- resistance. KO plants are very useful for the scientific community, since they allow for quantfication of the importance of different genes; i e if a plant lacking one particular protein grows significantly smaller than the wild type plant, the protein has an important function under these conditions. All plants covered by this application are "loss-of-function" mutants, such mutants are generated spontaneously in natural populations and would have been selected for if beneficial, so these mutants are likely to perform less well that the unmodified controls.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
In all cases, the sole function of the inserted fragment into the genome is to eliminate - in some cases just reducing - the expression of an endogenous Arabidopsis gene (plus to provide a marker for selection). No other function is introduced. As mentioned above, such mutants are generated continously in natural populations and would have been selected for if beneficial, so these mutants are likely to perform less well that the unmodified controls. We have performed similar experiments with many such lines in the past, and typically observed that the performed like wild type plants or worse. We can of course not exclude the possibillity that some of these lines will grow better that wild type controls and that this mutation for some reason have not appeared in the wild, but it unlikely.

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated T-DNA insertion

Experimental Release

1. Purpose of the release:
Basic research on Arabidopsis. As mentioned above, KO plants are standard tools for the scientific community and allow for quantfication of the importance of individlual genes. All plants covered by this application are "loss-of-function" mutants, and our research interest is to understand the functions of - more or less - all genes in Arabidopsis. We know from many years of studies that many loss-of-function mutants perform pretty OK under controlled conditions in climate chambers and/or greenhouses, but when challenged with natural conditons, the correct expression of all genes are typically necessat to sustain maximum growth and reproduction. Therefore, studies under natural conditions are essential complements to the endeavour to understand how plants live and growth, and how all genes function.

2. Geographical location of the site:
Umeå University. Sweden

3. Size of the site (m2):
Less than 20 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
To reduce or eliminate expression of any Arabidopsís proteins can not be expected to affect environment or human health.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Since all loss-of-function mutations of genes are likely to occur spontaneously in natural populations, all loss-of-function that confers a selective advantage would have already occurred spontasously and would have been selected for. Therefore, any mutation due to the insertion of a T-DNA or a transposon is highly unlikely to give a selectable advantage.

Brief description of any measures taken for the management of risks:
Our previously established protocol for management of the site (daily inspections, measures to eliminate cross-pollination and seed dispersal, waste management etc.) make it highly unlikely that and transgenic material will spread. Although Arabidopsis is a more or less obligate self-pollinator - and Arabidopsis does not grow in the region and it is doubtful that it could hybridize with any species found in the region - we nevertheless cover the field to prevent insect-born cross-pollination. All material - soil, plants etc - from the experimental area is treated like trangenic material, removed and distructed.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Our previously established protocol for management of the site (daily inspections, measures to eliminate cross-pollination and seed dispersal, waste management etc.) make it highly unlikely that and transgenic material will spread. Although Arabidopsis is a more or less obligate self-pollinator - and Arabidopsis does not grow in the region and it is doubtful that it could hybridize with any species found in the region - we nevertheless cover the field to prevent insect-born cross-pollination. All material - soil, plants etc - from the experimental area is treated like trangenic material, removed and distructed.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
28/06/2012 00:00:00
Remarks: