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Notification report


General information

Notification Number
B/SE/11/2397

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
22/02/2011

Title of the Project
Increased production of wood biomass by increased growth or improved drought stress tolerance

Proposed period of release:
01/08/2011 to 01/08/2016

Name of the Institute(s) or Company(ies)
SweTree Technologies AB, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-T89

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application consists of 14 different gene constructs. In each construct one gene from hybrid aspen or a populus orthologous gene from Arabidopsis thaliana has been up- or down- regulated. These constructs have been selected since they indicate improved growth characteristics and/or increased resistance to drought stress in greenhouse experiments. Five different vectors were used: Genes introduced from these are:1) p35S followed by gene of interest in two inverted copies with an intron in between, followed by a terminator T35S, neomycin phosphotransferase NPT-II resistance marker gene driven by the Nos promoter. 2) p35S followed by gene of interest and a terminator T35S, neomycin phosphotransferase NPTII resistance marker gene driven by Nos-promoter.3) p35S followed by gene of interest and a terminator T35S, hygromycin phosphotransferase gene (HPT/Hyg) resistance marker gene driven by Nos promoter.4) LMP1 promoter followed by gene of interest and terminator fom nopaline syntase (pANos), hygromycin phosphotransferase gene (HPT/Hyg) resistance marker gene driven by Nos promoter and bla (β-lactamase) with replicon from pBR322.5) LMP1 promoter followed by gene of interest and terminator from nopaline syntase (pANos), hygromycin phosphotransferase gene (HPT/Hyg) resistance marker gene driven by Nos promoter and bla (β-lactamase) with replicon from pBR322.The 14 gene constructs each contains one trait gene or trait gene fragment. These genes code for transcription factor proteins, zinc finger domain proteins, one protein involved in secondary metabolism, one protein involved in the vesicular transport mechanism and two proteins involved in signal transduction. The gene source is either Arabidopsis or hybrid aspen. The Arabidopsis genes all have homologous/ orthologous genes in hybrid aspen.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
See B2. The intended function is to increase growth and/or improve drought stress tolerance

6. Brief description of the method used for the genetic modification:
Agrobacterium mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind polinated and produce wind-dispersed seeds. Hybrid aspen can also propagate from suckers.

Experimental Release

1. Purpose of the release:
The purpose of the release of these trees is to study growth and physiological properties as well as wood properties to increase knowledge about growth and drought stress properties. In the future this experience may lead us to better yield varieties or varieties that give drought characteristics. These new tree varieties may be either GMO trees or trees developed using marker assisted breeding.The trees will be inspected regularly for variations in growth and morphology as well as biotic and abiotic stress tolerance. All changes will be quantified.Our intention is not to take these trees, based on the T89 clone of hybrid aspen, into a commercial product.

2. Geographical location of the site:
VĂ„xtorp in Laholms municipial

3. Size of the site (m2):
about 1,5 hectar

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Dnr 22-12395/09, 2010-04-29, The Department of Plant Physiology at Umeå University got approval for a field trial including genetically modifyed hybrid aspen last year where none of the genetic modifications are identical to the modifications in this application.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
We hope that some of the lines will show increased growth and/or increased resistance to drought stress in the field. This may lead to better tree varieties for increased wood, pulp and energy production using low input forest plantation systems.

Brief description of any measures taken for the management of risks:
Regular inspection for early detection of flower induction. It is possible to distinguish floral buds from vegetative buds before bud burst. If any line will show tendency of flowering all lines of corresponding construct will be eliminated. The site will be surrounded by a fence and a security zone will prevent suckers to spread out of or into the site. After harvest the remaining roots will be eliminated and the site will be inspected yearly for at least another three years.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Regular inspection for early detection of flower induction. It is possible to distinguish floral buds from vegetative buds before bud burst. If any line will show tendency of flowering all lines of corresponding construct will be eliminated. The site will be surrounded by a fence and a security zone will prevent suckers to spread out of or into the site. After harvest the remaining roots will be eliminated and the site will be inspected yearly for at least another three years.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
01/07/2011 00:00:00
Remarks: