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Notification report


General information

Notification Number
B/SE/11/12623

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
25/11/2011

Title of the Project
Increased production of wood biomass by increased growth 2.

Proposed period of release:
01/06/2012 to 01/06/2017

Name of the Institute(s) or Company(ies)
SweTree Technologies AB, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
hybrid aspensalicaceaepopuluspopulus tremula x populus tremuloides-Clone T89

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
This application consists of 7 different gene constructs. In each construct one gene from hybrid aspen has been up- or down- regulated. These constructs have been selected since they indicate improved growth characteristics in greenhouse experiments.
Four different vectors were used. Genes introduced from these are:
1) p35S followed by gene of interest in two inverted copies with an intron in between, followed by a terminator T35S, neomycin phosphotransferase NPT-II resistance marker gene driven by the Nos promoter.
2) p35S followed by gene of interest and a terminator T35S, neomycin phosphotransferase NPTII resistance marker gene driven by Nos-promoter.
3) LMP1 promoter followed by gene of interest and terminator fom nopaline syntase (pANos), hygromycin phosphotransferase gene (HPT/Hyg) resistance marker gene driven by Nos promoter and bla (β-lactamase) with replicon from pBR322.
4) LMX5 promoter followed by gene of interest and terminator from nopaline syntase (pANos), hygromycin phosphotransferase gene (HPT/Hyg) resistance marker gene driven by Nos promoter and bla (β-lactamase) with replicon from pBR322.
The 7 gene constructs each contains one trait gene or trait gene fragment. These genes code for transcription factor proteins, one protein involved in vesicular transport mechanism and one protein involved in calcium ion signaling system. The gene source is hybrid aspen.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The 7 gene constructs each contains one trait gene or trait gene fragment (GOI). These genes code for transcription factor proteins, one protein involved in vesicular transport mechanism and one protein involved in calcium ion signaling system. The source of all GOIs is hybrid aspen. The intended function of the GOI is to increase growth.
Genes and fragments included in the T-DNA that is transferred into the plant from each vector are:
Gene/fragment (source)
1) Promoter, p35S (Cauliflower mosaic virus), Ω leader (Tobacco mosaic virus), attB1 recombination site (Gateway Technology), RNAi fragment, GOI (Hybrid aspen), attB2 recombination site (Gateway Technology), Intron (Arabidopsis), attB2 recombination site (Gateway Technology), RNAi fragment, GOI (Hybrid aspen), attB1 recombination site (Gateway Technology), Terminator t35S (Cauliflower mosaic virus), Promoter, pnos (Agrobacterium), Kanamycin marker gene, npt-II (E. coli), Terminator, tnos (Agrobacterium).
2) Terminator t35S (Cauliflower mosaic virus), attB2 recombination site (Gateway Technology), Gene of interest, GOI (Hybrid aspen), attB1 recombination site (Gateway Technology), Ω leader (Tobacco mosaic virus), Promoter, p35S (Cauliflower mosaic virus), Promoter, pnos (Agrobacterium), Kanamycin marker gene, npt-II (E. coli), Terminator, tnos (Agrobacterium).
3) Terminator, tnos (Agrobacterium), Hygromycin marker gene, hpt (E. coli), Promoter, pnos (Agrobacterium), Linker sequence (E. coli), Terminator, tnos (Agrobacterium), attB2 recombination site (Gateway Technology), Gene of interest, GOI (Hybridasp), attB1 recombination site (Gateway Technology), Promoter, LMP1 (Hybrid aspen), β-lactamase marker gene for bacteria, including pP3 (Salmonella), BOM site + origin of replication ColE1 for bakteria (E. coli).
4) Terminator, tnos (Agrobacterium), Hygromycin marker gene, hpt (E. coli), Promoter, pnos (Agrobacterium), Linker sequence (E. coli), Terminator, tnos (Agrobacterium), attB2 recombination site (Gateway Technology), Gene of interest, GOI (Hybrid aspen), attB1 recombination site (Gateway Technology), Promoter, LMX5 (Hybrid aspen), β-lactamase marker gene for bakteria, including pP3 (Salmonella), BOM site + origin of replication ColE1 for bacteria (E. coli).


6. Brief description of the method used for the genetic modification:
Agrobacterium mediated transformation

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Hybrid aspen is wind polinated and produce wind-dispersed seeds. Hybrid aspen can also propagate from suckers.

Experimental Release

1. Purpose of the release:
The purpose of the release of these trees is to study growth and physiological properties as well as wood properties for increased knowledge about how these genes affect growth properties. In the future this experience may lead us to better yield varieties. These new tree varieties may be either GMO trees or trees developed using marker assisted breeding.
The trees will be inspected regularly for variations in growth and morphology as well as biotic and abiotic stress tolerance. All changes will be quantified. If the change in overall morphology or biotic/ abiotic stress is significant compared to wild type plants we will decide whether further investigation is nessesary.
These trees, based on the T89 clone of hybrid aspen, will not be commercialised.


2. Geographical location of the site:
VĂ„xtorp in Laholms municipia

3. Size of the site (m2):
about 5000m2

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The aim of the release of these GM-plants is to show improved growth properties in the field for some of the lines tested. High growth in aspen trees may be considered as an selective advantage in natural environments. The source of the modified genes is hybrid aspen. The genetic variation, and variations in growth among different natural populations of aspen or Populus species is high and we assess that none of the improvements will lead to increased environmental risk. In addition we will perform reliable control measures to avoid dissemination of the GM-plants (see E).
Local environmental impact on the site is in the same level as plantations of non transgenic hybrid aspen. Evaluation of the proteins, over expressed in the GM-plants, did not show any significant risk of allergenic cross-reactivity, according to FAO/WHO 2001.
All modifications in the application include nptII or hpt antibiotic marker gene, which are expressed in the GM-plant. The resistance to antibiotic will result in environmental benefits of the plant, only in the presence of high levels of the antibiotic compound, which is not the case in nature. Accordingly we assess that the presence of the marker genes do not add any environmental impact.
Positive environmental effects of this trial are that it will lead an increased understanding of biomass production and possible direct ways to increase biomass production. Increased biomass production could lead to many positive effects for the environment, for example a reduced dependence of fossil carbon for energy and chemicals.


Brief description of any measures taken for the management of risks:
Regular inspection for early detection of flower induction. Normaly aspen flower within 8-20 years, well after this experiment will be terminated. It is possible to distinguish floral buds from vegetative buds before bud burst. If any line will show tendency of flowering all lines of corresponding construct will be eliminated. The site will be surrounded by a fence and a security zone will prevent suckers to spread out of or into the site. After harvest the remaining roots will be eliminated and the site will be inspected yearly for at least another three years.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Not known