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Notification report


General information

Notification Number
B/SE/08/13001

Member State to which the notification was sent
Sweden

Date of acknowledgement from the Member State Competent Authority
23/12/2008

Title of the Project
Notification B for the field testing of spring oilseed rape (Brassica napus) genetically modified for improved oil composition in the seed

Proposed period of release:
01/04/2009 to 31/12/2013

Name of the Institute(s) or Company(ies)
Plant Science Sweden AB, Herman Ehles väg 2-4
SE-268 31 Svalöv
Sweden;


3. Is the same GMPt release planned elsewhere in the Community?
Yes:


Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
canolabrassicaceaebrassicabrassica napusnapus (syn. oleifera)

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- improved oil composition in the seed
- Marker gene ahas (tolerance to Imidazolinones) as selectable marker gene to identify transgenic cells in tissue culture


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Trait genes used:
- Fungal-derived ∆5-Desaturase
- Algae-derived ∆6-Desaturases
- Fungal-derived ∆6-Desaturase
- Fungal-derived ∆12-Desaturase
- Plant-derived ∆15-Desaturases
- Algae-derived ∆6-Elongase
- Fungal-derived ω3-Desaturase

Construct Gene combination
1 Fungal-derived ∆5-Desaturase, Fungal-derived ∆6-Desaturase, Algae-derived ∆6-Desaturase, Fungal-derived ∆12-Desaturase, Plant-derived ∆15-Desaturase, Fungal-derived ω3-Desaturase, Algae-derived ∆6-Elongase

2 Fungal-derived ∆5-Desaturase, Fungal-derived ∆6-Desaturase, Algae-derived ∆6-Desaturase, Fungal-derived ∆12-Desaturase, Plant-derived ∆15-Desaturase, Fungal-derived ω3-Desaturase, Algae-derived ∆6-Elongase

The trait genes are combined with the following promoters:
- the napin promoter from Brassica napus (p Napin)
- the promoter of a 2S storage protein from Linum usitatissinum (p Conlinin)
- the promoter of a Sucrose Binding Protein of Vicia faba (p SBP)
- the promoter of a seed protein gene from Vicia faba (p USP)
- the promoter of the acyl-carrier protein gene from Brassica napus (p ACP)
- the promoter of the fatty acid elongase gene 1 from Brassica napus (p Fae1)

Terminators used for the termination of the expression of the trait genes are:
- the terminator of the 35S gene from the Cauliflower mosaic virus (t CaMV35S)
- the terminator of the octopine synthase gene from Agrobacterium tumefaciens (t OCS)
- the terminator of the cathepsin D inhibitor gene from Solanum tuberosum (t StCat)
- the terminator of the small subunit of the ribulose-1,5-bisphosphate carboxylase gene from Pisum sativum (t E9)
- the terminator of the acyl-carrier protein gene from Brassica napus (t ACP)
- the terminator of the fatty acid elongase gene 1 from Brassica napus (t Fae1)

The selection cassette contains following elements:
- the ubiquitin promoter from Petroselinum crispum (p-PcUbi),
- nos (nopaline synthase) terminator from Agrobacterium tumefaciens
- the mf-SceI1 sense and antisense excision recognition sites


6. Brief description of the method used for the genetic modification:
An Agrobacterium based transformation system was used for transformation of spring oilseed rape B878.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
The specific purpose of the release is the evaluation of the performance of the added trait under field conditions for research purposes.

2. Geographical location of the site:
Municipalities of Eslöv, Svalöv, Klippan, Kristianstad and Vara.

3. Size of the site (m2):
Total Area will not exceed 15 ha (150 000m2) per year

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
No previous releases have been carried out.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified oilseed rape lines show an altered fatty acid composition in seeds towards very long-chain polyunsaturated fatty acids. The plant material also shows tolerance during the selection process to the imidazolinone herbicides in tissue culture.
The added traits are not likely to have a critical influence under field conditions on any important competitive factors such as pollen production/dispersal, fertility or compatibility, nectar production, seed dispersal or general fitness. Based on observations in the greenhouse the genetically modified oilseed rape lines do not differ from the parental control lines in their morphology, flowering time, germination rate or kernel weight. No difference regarding persistence in agricultural habitats or invasiveness in natural habitats or persistence in agricultural habitats compared to conventional oilseed rape is expected.
Considering the very low likelihood of the introduced traits to confer increased fitness or the GM oilseed rape lines in any other way to become more persistent than the parental Brassica napus plant material in agricultural habitats or more invasive in natural habitats, the overall impact is expected to be negligible.
Any effects from the ahas gene on non-target organisms are not expected and have not been reported from other field trials harbouring this trait. The overall impact is expected to be negligible.


Brief description of any measures taken for the management of risks:
An isolation distance of 800m to other Brassica crops will be observed. Planting and harvesting equipment will be cleaned on site to prevent the dispersal of GM seeds. There will be no oilseed rape or any other crossable crop cultivation on the release area the following four years after the release. Potential volunteers will be monitored and removed according to conventional agricultural practice. During the release the trial site will be monitored at defined intervals.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
12/05/2009 00:00:00
Remarks: