Member State to which the notification was sent
Date of acknowledgement from the Member State Competent Authority
Title of the Project
Effects of the transgenic apple and pear rootstocks on growth characteristics of five apple cultivars and three pear cultivars grafted onto them in comparison with the untransformed apple rootstocks M26 and M9 and the pear rootstock BP10030.
Proposed period of release:
01/04/2004 to 31/12/2004
Name of the Institute(s) or Company(ies)
Swedish University of Agriculture Sciences, Department of Crop Science, ;
3. Is the same GMPt release planned elsewhere in the Community?
Has the same GMPt been notified elsewhere by the same notifier?
Genetically modified plant
Complete name of the recipient or parental plant(s)
Rootstocks M26 & Rootstock M9
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The rooting ability of the transgenic clones was significantly increased compared with the non-transformed control plant. In vitro propagated shoots of transformed M26 and BP10030 rooted to 100%, and 86% for M9, but no roots were observed for the non-transformed rootstocks on the auxin-free rooting medium. Stem cuttings of the transgenic apple and pear rootstocks rooted up to 100%, but only 44% for non-transformed M26 and 5% for non-transformed BP10030 without auxin treatment.
3. Type of genetic modification:
In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
1) The rolB gene is from Agrobacterium rhizogenes and increases the rooting ability of the transgenic plants.
2) The nptII gene is from Escherichia coli and is a selection gene.
3) The gus gene is from Escherichia coli and is a reporter gene.
6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated gene transfer was used for the genetic modification.
7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
1. Purpose of the release:
The purposes of the field trial are:
1) to evaluate the effects of the transgenic rootstocks on growth, flowering, fruit setting and fruit quality of the three cultivars grafted onto them in comparison with the untransformed control rootstock for commercial potential.
2) to evaluate the stability of the modified trait (rooting ability).
3) to evaluate the rooting ability of the transgenic rootstocks themselves by stooling.
2. Geographical location of the site:
Alnarp in Lomma community
3. Size of the site (m2):
4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The transgenic rootstocks of M26 and M9 have been released in 2000-2004 and this application for apple rootstocks is the continuation of the previous field trial. The rootstocks are growing in the field and have not flowered and will not be allowed to flower. No environmental and human health impacts have been observed.
Environmental Impact and Risk Management
Summary of the potential environmental impact from the release of the GMPts:
The increased rooting ability of transformed rootstocks may increase the anchorage of grafted trees. This may in turn reduce the use of support to the trees and thus reduce the production cost. The transgenic plants used as rootstocks for the cultivars will not be able to flower, so there is no risk for them to spread pollen to other plant species nearby. The non-grafted transgenic rootstocks will be evaluated by stooling. In this case the trees will be cut down every year and will not flower.
Brief description of any measures taken for the management of risks:
The trees will be uprooted and burnt after the field trial has been finished. The field will be ploughed to eliminate all roots that may be left in the soil.
Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
European Commission administrative information
Consent given by the Member State Competent Authority: