General informationNotification NumberB/SE/04/1101Member State to which the notification was sentSwedenDate of acknowledgement from the Member State Competent Authority16/02/2004Title of the ProjectPotato starch with increased amylose contentProposed period of release:01/04/2004 to 31/10/2008Name of the Institute(s) or Company(ies)Plant Science Sweden AB, Herman Ehles väg 2-4
SE-268 31 Svalöv
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:- inhibition of starch branching enzymes be1 and be2, which yields a higher fraction of amylose in the starch through a reduced branching of amylopectin
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
|potato||solanaceae||solanum||solanum tuberosum||tuberosum||P763 and P800|
- Marker gene AHAS (imidazolinone tolerance) as selection gene to identify transgenic cells in tissue cultureGenetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:T-DNA borders, pTiT37, for incorporation into plant chromosome.Recombinant AHAS gene, A. thaliana, imidazolinone tolerance in plant material.Promoter and polyadenylation sequence from nopaline synthase gene, A. tumefaciens, gene regulation. Inverted repeat fragments of be1 and be2, S. tuberosum, inhibition of amylopectin biosynthesis.GBSS promoter, S. tuberosum, expression of inverted repeat fragments.6. Brief description of the method used for the genetic modification:A. tumefaciens mediated transformation of cut leaf discs from S. tuberosum.Binary vector system has been used.Transgenic tissue has been selected on medium containing imazamox. A. tumefaciens has been killed with Claforan.7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:Not applicableExperimental Release1. Purpose of the release:- Investigate expression and stability of modified traits
- Identify and eliminate morphologically divergent clones
- Investigate the agricultural and industrial value of the modified lines
- Produce potato tubers for laboratory analyses
- Produce seed potatoes for future trials2. Geographical location of the site:Landskrona, Halmstad, Kristianstad, Alvesta, Skara, Luleå och Robertsfors communities3. Size of the site (m2):Max 50 000 m2 in total4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:Modified potato lines with AHAS as marker gene (imazamox tolerance gene) grown in field test 2002 and 2003.
Modified potato lines with inhibition of starch branching enzymes and thus increased amylose content in field tests yearly 1998-2003.
No impact on the environment or human health has been observed during these trials.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:There is no reason to assume that the transgenic lines will behave in and interact with the environment differently compared to their mother varieties.
There are no experiences that imply that an altered carbohydrate composition or carbohydrate content could influence frost tolerance neither of plants nor tubers. Neither high starch content (starch potatoes) nor altered sugar content/composition (chip potatoes) have such effects. Earlier genetic modifications with increased amylose content have not been shown to influence the frost tolerance.
The introduced selection marker gene gives an increased tolerance to herbicides of imidazolinone type. This gives no selective advantage compared to other potatoes outside the agricultural habitat. As herbicides of imidazolinone type are not used in Sweden no selective advantage in cultivated fields is given either.Brief description of any measures taken for the management of risks:- isolation distance: new transformants: 20 m
- careful harvest to avoid volunteers
- transportation in specific vehicles, closed containers or double sacks
- adjusted crop rotation: new transformants: fallow field the following year, no potato crop for one additional year
- destruction of waste materialSummary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:Not applicableFinal reportEuropean Commission administrative informationConsent given by the Member State Competent Authority:Yes10/05/2004 00:00:00Remarks: