Member State to which the notification was sent
Date of acknowledgement from the Member State Competent Authority
Title of the Project
Improved starch biosynthesis
Proposed period of release:
15/04/2003 to 01/10/2007
Name of the Institute(s) or Company(ies)
Plant Science Sweden AB, Herman Ehles väg 2-4
SE-268 31 Svalöv
3. Is the same GMPt release planned elsewhere in the Community?
Has the same GMPt been notified elsewhere by the same notifier?
Genetically modified plant
Complete name of the recipient or parental plant(s)
P107, P708, P86, AM99-2003. AM99-2003 is a transgenic potato line which has been modified for increased amylose content.
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- Antisense constructs for inhibition of StGH1 and StGH2 respectively for the purpose of investigating the impact of each genes impact on starch biosynthsis, reduction of dry matter content in the tuber
- Gene constructs for over-expression of StGH1 and StGH2 respectively for the purpose of investigating the impact of each genes impact on starch biosynthsis, increase of dry matter content in the tuber
- Marker gene AHAS (imidazolinone tolerance) as selection gene to identify transgenic cells in tissue culture
- Marker gene nptII (kanamycinresistance) as selection gene to identify transgenic cells in tissue culture
- Antisense construct for the inhibition of starch branching enzymes Sbe1 and Sbe2 yields a higher fraction of amylose in the starch through a reduced branching of amylopectin
3. Type of genetic modification:
In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
T-DNA borders, pTiT37, for incorporation in plant chromosome.
Recombinant AHAS-gen, A. thaliana and A. tumefaciens; imidazolinon tolerance in plant material.
Recombinant nptII-gen, Tn5, A. tumefaciens; kanamycin resistance in plant material.
Recombinant StGH1-gene, S. tuberosum, inhibition of endogenous StGH1-gene.
Recombinant StGH2-gene, S. tuberosum, inhibition of endogenous StGH2-gene.
Recombinant StGH1-gene, S. tuberosum, over-expression of endogenous StGH1-gene.
Recombinant StGH2-gene, S. tuberosum, over-expression of endogenous StGH2-gene.
The products of genes StGH1 and StGH2 with origin Solanum tuberosum contain the same catalytic domain. The function of this catalytic domain is to synthesise short glucose chains (DS 6-8) with α-1,4 linkage between the glucose units.
6. Brief description of the method used for the genetic modification:
A. tumefaciens mediated transformation of cut leaf discs from S. tuberosum.
Binary vector system has been used.
Transgenic tissue has been selected on medium containing kanamycin or imazamox.
A. tumefaciens has been killed with Claforan.
7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
1. Purpose of the release:
- Investigate expression and stability of modified traits
- Identify and eliminate morphologically diverging clones
- Investigate the agricultural and industrial value of the modified lines.
- Produce potato tubers for labaratory analyses.
- Produce seed potatoes for future trials.
2. Geographical location of the site:
Field trial in Landskrona kommun (Axeltofta gård, Häljarp), Skåne län
3. Size of the site (m2):
4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Modified potato lines with AHAS as marker gene (imazamox tolerance gene) grown in field test 2002.
Modified potato lines with nptII as marker gene (kanamycin resistance gene) grown in field tests 1992-2002.
Modified potato line AM99-2003 (increased amylose content) grown in field tests 2000-2002.
Environmental Impact and Risk Management
Summary of the potential environmental impact from the release of the GMPts:
There is no reason to assume that the transgenic lines will behave in and interact with the environment differently compared to their mother varieties.
Brief description of any measures taken for the management of risks:
- isolation distance: new transformants: 100 m
- careful harvest to avoid volunteers
- transportation in specific vehicles, closed containers or double sacks
- adjusted crop rotation: new transformants: fallow field the following year, thereafter no potato crop for two years
- destruction of waste material
Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
European Commission administrative information
Consent given by the Member State Competent Authority: