General informationNotification NumberB/PL/08/02-03Member State to which the notification was sentPolandDate of acknowledgement from the Member State Competent Authority1/14/2008Title of the ProjectQuality evaluation of some chosen cucumber transgenic lines with taumatin geneProposed period of release:5/15/2008 to 9/30/2012Name of the Institute(s) or Company(ies)Warsaw University of Life Sciences (SGGW), Faculty of Horticulture and Landscape Architecture, Department of Vegetable and Medicinal Plants;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:A commercially important gene of taumatin II that codes the monomeric protein raises special interest due to its nature of evoking the sweet taste sensation in human. The gene undergoes the expression only in the fruit of Thaumatococcus danielli – the African plant from which it was obtained.
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
The promoter originates from cauliflower mosaic virus (35S RNA gene promoter from CaMV). This is the only virus-derived genetic material, not required for the transformation, and the virus itself has not been yet reckoned as a secondary threat. T-DNA region of pRUR528 plasmid is a derivative of pROK2 of E. coli plasmid. Beyond the T-DNA region of pRUR528 plasmid there is gene npt II, being under control of bacterial promoter.Genetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:Vector transformation
Origin /organism/: Echerichia coli
The promoter originates from cauliflower mosaic virus (35S RNA gene promoter from CaMV). This is the only virus-derived genetic material, not required for the transformation, and the virus itself has not been yet reckoned as a secondary threat. The promoter (CaMV35) and terminator (nos t) are joined to cDNA of taumatin II, supported by dr A.M. Ledeboer (Unilever the Netherlands). In T-DNA region of pRUR528 plasmid there is located gene npt II remaining under control of regulatory factors of nopaline synthase (nos) gene what allows the selection of the cells transformed. These are the only genes present in the construct used for the transformation and they come to expression in the plant cell.6. Brief description of the method used for the genetic modification:Agrobacterium tumefaciens - mediated transformation.
The genetic construct of pRUR528 was carried on this plasmid to the genome of the inbred Borszczagowski line by the transferring of plasmid DNA to leaf tissue and then the regenerating of plants via tissue culture. Four lines (T2 generation) originating from 3 primary transformation events were selected for the study.Experimental Release1. Purpose of the release:The intentional release will be performed during field cultivation in order to evaluate the transgenic lines of different levels of transgene expression in plants, showing both diverse taste of fruit and resistance to downy mildew.2. Geographical location of the site:Experimental field of Horticulture and Landscape Architecture Faculty.
Plot number 10628/10628
44 str Vogla, Warsaw, Poland3. Size of the site (m2):150 m24. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:The studies concerning cucumber, carried out in 2001-2007, did not bring about any threats to human health or environment what indicates that the growth and development of the cucumber with taumatin II proceed the same as in nontransgenic lines of the speciesEnvironmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:Experiments concerning genetic modification of cucumber make no potential threat to the environmentBrief description of any measures taken for the management of risks:Seedlings will be produced in a restricted glasshouse unit, allowed only for this purpose. 1000 m isolation distance from other cucumber cultivations. The experimental area will be monitored according to the methodology, once a year after finishing the study. During the following seasons other plant species will be grown on the experimental field and herbicides against dicotyledonous weeds will be applied.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:Transgenic cucumbers are not a real threat to human and environment. The studies concerning cucumber, carried out in 2001-2007, did not bring about any threats to human health or environment what indicates that the growth and development of the cucumber with taumatin II proceed the same as in nontransgenic lines of the species. The presence of the commercial gene in form of cDNA taumatin II is neither hazardous as the gene is derived from fruits of African plant – Thaumatococus danielli, eaten for hundred years by native people in Africa. Taumatin has been used in food industry as a sugar substitute in diabetic products and is recognized a dietary-safe protein. Therefore the genetic modification do not affect adaptation traits. Alike nontransgenic cucumbers the studied lines do not overwinter under Polish weather conditions. Self−seedlings are therefore not a problem. A genetically modified cucumber is not capable of colonizing any natural habitats in our country.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:No7/15/2009 12:00:00 AMRemarks: