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Notification report

General information

Notification Number
B/PL/04/02-02

Member State to which the notification was sent
Poland

Date of acknowledgement from the Member State Competent Authority
10/05/2004

Title of the Project
Transgenic flax cultivation

Proposed period of release:
15/04/2006 to 15/09/2010

Name of the Institute(s) or Company(ies)
Institute of Biochemistry and Molecular Biology ,University of Wroclaw, Poland, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
flax	linaceae	linum	linum usitatissimum		Nike, Linola

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Improvement of thermoplastic properties and elasticity of flax fibres. Increasing of antioxidant capacity of flaxseeds.
Marker genes npt II (kanamycine resistance) i hyg (hygromycin resistance).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Genes encoding beta-ketothiolase (phb A), acetoacetyl-CoA reductase (phb B) and PHB synthase (phb C)- enzymes of PHB biosynthesis pathway; genes derived from Ralstonia eutropha.
Genes: chalcone synthase (CHS), chalcone isomerase (CHI) and dihydroflavonone reductase (DFR)- were isolated from Petunia hybrida.

6. Brief description of the method used for the genetic modification:
The explants of flax hypocotyls and cotyledons were transformed by incubation with suspension of recombined Agrobacterium tumefaciens.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not concerned

Experimental Release

1. Purpose of the release:
The aim of the project is verification of genetically improved transgenic plants properties in field trial; generated plants were enriched in biodegradable plastic (polyhydroxybutyrate). The goal is also to verify in field trial transgenic plants with properties of high resistance to pathogen infection and higher antioxidant capacity in seeds resulted from flavonoids overproduction.

2. Geographical location of the site:
Wroclaw -Karlowice, plot. nr 26, AM-13, experimental fields belonging to University of Wroclaw, Poland

3. Size of the site (m2):
400

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The performed previous releasing took place on the same experimental field and no uncontrolled spread over of GMO was observed and also another disadvantageous changes in environment were not notified.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Transgenic flax containing polyhydroxybutyrate has changed thermoplastic properties, which leads to improvement of fibre quality. The enlarging of flavonoids content caused increase in pathogen resistance of plants and protect unsaturated fatty acids from oxidation.
Obtained transgenic plants can not have selective dominance in natural environment.

Brief description of any measures taken for the management of risks:
During experiment the GMO trials will be controlled every week.
Every year after the end of experiment the field will be monitored at least once per month.
In case of any negative influence on natural environment the cultivation will be destroyed by herbicide treatment.
The experimental field is secured by 4 m fence.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We do not expect any influence on natural environment and human health, it is unexpected any consequence of release of transgenic plant to environment.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
08/01/2007 00:00:00
Remarks: