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Notification report


General information

Notification Number
B/NL/12/L02

Member State to which the notification was sent
Netherlands

Date of acknowledgement from the Member State Competent Authority
29/10/2012

Title of the Project
Selection of genetic modified potato plants for late blight resistance III

Proposed period of release:
01/01/2013 to 31/12/2022

Name of the Institute(s) or Company(ies)
Wageningen University, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosum

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The GM-potatoes are characterized by a higher level of late blight resistance. Depending on the construct(s), the GM-potatoes may contain the selection marker genes NPTII (kanamycine) and/or HygR (hygromycine).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Depending on plasmid and construct different combinations of the following fragments are used:
-T-DNA borders from Agrobacterium tumefaciens for stable incorporation into the plant genome.
-The NPTII gene from Klebsiella pneumonia, to be used as selection marker.
-The HygR gene from Escherichia coli, to be used as selection marker.
-Nopaline synthase promoter and terminator from Agrobacterium tumefaciens for regulation of selection marker expression.
-Terminator Tg7 from Agrobacterium tumefaciens for regulation of selection marker expression.
-Cauliflower mosaic virus 35S gene terminator and promotor for expression of marker genes and late blight resistance genes.
-Late blight resistance genes (Rpi-genes) from Solanaceae.
-Promotors and terminators of Rpi-genes for regulation of different Rpi-genes.


6. Brief description of the method used for the genetic modification:
T-DNA was introduced into potato genotypes by agrobacterium-mediated gene transfer technology using a binary vector system.

Experimental Release

1. Purpose of the release:
-Production of seed tubers for future field trials
-Evaluation of late blight resistance under field conditions
-Evaluation of the agricultural value of modified genotypes


2. Geographical location of the site:
Municipalities: Borger-Odoorn, Eemsmond, and Wageningen. All municipalities are located in The Netherlands.

3. Size of the site (m2):
The size per site will be variable. The maximum size per site is 10,000 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Similar GM-plants were grown from 2008 until 2012 (licenses IM 07-001 and IM 09-002) on the same locations. Compared to classical breeding stocks, no deviations of any kind were observed. Cultivars, derived by classical breeding methods, containing late blight resistance genes (Rpi-genes) are being cultivated for several decades without any negative impact on the environment or human health.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced resistance will not confer an increased selective advantage in natural environments compared to the effect of late blight resistance genes present in potato cultivars.

Brief description of any measures taken for the management of risks:
All handling of GM-plants and GM-fields will be done according to good agricultural practices.
-Transportation of potatoes will be done in boxes or bags in open containers.
-Crop rotation: no potato crop for at least the following two years.
-Destruction of volunteers.
-destruction of waste material.


Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known