General informationNotification NumberB/NL/11/05Member State to which the notification was sentNetherlandsDate of acknowledgement from the Member State Competent Authority07/11/2011Title of the ProjectApplication for the release into the environment of potato lines with improved resistance to Phytophthora infestans according to the Genetically Modified Organisms Decree,2012 - 2018Proposed period of release:01/01/2012 to 31/12/2018Name of the Institute(s) or Company(ies)BASF Plant Science GmbH, ;
3. Is the same GMPt release planned elsewhere in the Community?Yes: Germany; Sweden; Has the same GMPt been notified elsewhere by the same notifier?YesIf yes, notification number(s): B/CZ/07/01; B/DE/05/174; B/DE/06/183; B/DE/07/191; B/GB/06/R42/01; B/NL/05/03; B/NL/07/07; B/SE/05/8615; Genetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:-improved resistance to Phytophthora infestans
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
-marker gene ahas (tolerance to Imidazolinones) as selection gene to identify transgenic cells in tissue cultureGenetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:- T-DNA borders, pTiT37, from Arabidopsis thaliana for stable incorporation into plant chromosome.
- acetohydroxyacid synthase gene (ahas, EC 126.96.36.199, 2013 base pairs, mutation S653N) from Arabidopsis thaliana, conferring imidazolinone tolerance in plant material.
-Promoter and terminator from nopaline synthase gene, Agrobacterium tumefaciens, gene regulation.
-Resistance genes Rpi-blb1 and Rpi-blb2 from Solanum bulbocastanum, with endogenous promoters and terminators for improved resistance to Phytophthora infestans.6. Brief description of the method used for the genetic modification:Plasmid-derived DNA was introduced into the potato lines by Agrobacterium-mediated gene transfer technologyExperimental Release1. Purpose of the release:- Produce seed potatoes for future trials
- Produce plant material for further analyses
- Investigate the general agricultural value of the modified lines2. Geographical location of the site:The experimental fields will be located in the community of Steenbergen (province Noord-Brabant)3. Size of the site (m2):The maximum area for the release will be 10.000 m2.4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:Except the better resistance against Phytophthora infestans there were no deviations of the GM potato lines in comparison to the non-GM plots. During these trials no unforeseen effects as compared to conventional potato varieties have been observed.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The genetically modified potato lines contain two NBS-LRR-genes, Rpi-blb1 and Rpi-blb2, from Solanum bulbocastanum for conferring improved resistance to Phytophthora infestans. Many conventional potato varieties also contain additional NBS-LRR-genes that have been introgressed from wild Solanum species. An intended effect of the introduced trait is an increased survivability in potato fields exposed to P. infestans. This possible selective advantage, however, is of importance only in the agricultural field, and will not improve the survivability in the surrounding environment. The reduced need for fungicides on these lines can easily be identified as an environmental benefit.
The ahas gene expressed in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture. This confers no selective advantage in the field since Imidazolinone herbicides are not approved for use on crops in NL and since no field tolerance is expected in the potato plants. No difference with respect to persistence in agriculturally utilised habitats or invasiveness into natural habitats as compared to conventional potato varieties is expected. Through the measures which are taken during the release, distance from or absence of conventionally cultivated potatoes or wild species, the possibility of any gene transfer can be virtually ruled out. Even in the very improbable event that pollen were to be transferred to genetically unmodified potato plants, no consequences are to be expected, since potato propagation conventionally takes place via tubers and not via seeds. The interactions of the genetically modified potato line with non-target organisms and the effects resulting from this will be comparable to those with conventional potato varieties. Furthermore, no toxic or allergenic effects are expected on the basis of the improved resistance to P. infestans or the expressed AHAS protein. No effects on biogeochemical processes are expected, other than those that apply also to conventional potatoes.Brief description of any measures taken for the management of risks:- a distance to other commercially grown potatoes of 10 m will be kept
- transportation in closed containment
- careful harvest to avoid volunteers
- destruction of waste material
- no potato crop for the following year to allow volunteer monitoring
- the year following the field trial emerging volunteers will be destroyed by mechanical or herbicide treatmentFinal report-European Commission administrative informationConsent given by the Member State Competent Authority:Not known