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Notification report


General information

Notification Number
B/NL/10/06

Member State to which the notification was sent
Netherlands

Date of acknowledgement from the Member State Competent Authority
22/11/2010

Title of the Project
Potato late blight control strategies and monitoring of Phytophthora infestans virulence

Proposed period of release:
01/04/2011 to 31/12/2021

Name of the Institute(s) or Company(ies)
Stichting Dienst Landbouwkundig Onderzoek (DLO), Stichting Dienst Landbouwkundig Onderzoek (DLO) in particular Praktijkonderzoek Plant & Omgeving / Plant Research International (PPO/PRI);


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumstarch production and consumption

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The GM-potato is characterized by a higher level of late blight resistance. Depending on the construct, the GM-potato contains the selection marker gene NPTII or is marker free.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
In this project different constructs are used consisting of different combinations of the following fragments:
- T-DNA borders from Agrobacterium tumefaciens for stable incorporation into the plant genome.
- The NPTII gene from Escherichia coli, to be used as selection marker.
- Nopaline synthase promoter from Agrobacterium tumefaciens for regulation of NPTII.
- Nopaline synthase terminator from Agrobacterium tumefaciens for regulation of NPTII.
- Terminator Tg7 from Agrobacterium tumefaciens for regulation of NPTII expression.
- Rpi-genes, including original promoter and terminator, from tuber bearing Solanum species for late blight resistance.


6. Brief description of the method used for the genetic modification:
T-DNA was introduced into potato cultivars by Agrobacterium-mediated gene transfer technology using a binary vector system.

Experimental Release

1. Purpose of the release:
The purpose of the experiments is twofold:
1) Mixtures of true to type, resistant (R) and susceptible (S) plants are studied as a model for R/R mixtures after break through of one of the Rpi-genes or Rpi-gene cassettes. The purpose is to establish relationships between the mixing ratio of resistant/susceptible and the resulting (beneficial) spatial and temporal delay of epidemic progress. The results give insight in the added value of- and ideal composition of true to type dynamic cultivars and the most durable way to deploy them in time and space.
2) Monitoring of the P. infestans population for adaptation (virulence) to newly introduced Rpi-genes and Rpi-gene cassettes. This information is crucial for strategies for durable deployment of Rpi genes.


2. Geographical location of the site:
Municipalities: Borger Odoorn, Lelystad, Wageningen, Venray and Binnenmaas.

3. Size of the site (m2):
The size per site and per year will be variable. The maximum size per site per year is 10,000 m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Similar GM-plants were grown in 2008 and 2009 under license IM 07-001. GM-plants were compared to classical breeding stocks. No deviations of any kind were observed.
Potato cultivars, produced through classical breeding methods, containing late blight resistance genes (Rpi-genes) have been cultivated for several decades without any negative impact on the environment or human health.


Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced resistance to potato late blight will not confer an increased selective advantage in natural environments as compared to the effect of late blight resistance genes present in commercial potato cultivars.

Brief description of any measures taken for the management of risks:
All handling of GM-plants, GM crops and GM fields will be done according to good agricultural practices.
- Crop rotation: no potato crop the following two years.
Monitoring will take place with a minimum of one time per month during the growing season and next spring/summer.- Transportation of potatoes will be done in boxes or bags in closed containers.
- Destruction of volunteers.
- Destruction of collected GM waste material by steaming >4 hrs or autoclaving


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
All handling of GM-plants, GM crops and GM fields will be done according to good agricultural practices.
- Crop rotation: no potato crop the following two years.
Monitoring will take place with a minimum of one time per month during the growing season and next spring/summer.- Transportation of potatoes will be done in boxes or bags in closed containers.
- Destruction of volunteers.
- Destruction of collected GM waste material by steaming >4 hrs or autoclaving


Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known