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Notification report


General information

Notification Number
B/NL/10/05

Member State to which the notification was sent
Netherlands

Date of acknowledgement from the Member State Competent Authority
02/11/2010

Title of the Project
Scab resistant cisgenic apples

Proposed period of release:
01/04/2011 to 01/04/2021

Name of the Institute(s) or Company(ies)
Stichting Dienst Landbouwkundig Onderzoek (DLO), Stichting Dienst Landbouwkundig Onderzoek (DLO) in particular Praktijkonderzoek Plant & Omgeving / Plant Research International (PPO/PRI);


3. Is the same GMPt release planned elsewhere in the Community?
Not known

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
applerosaceaemalusmalus domesticagala

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Resistance to the fungus Venturia inaequalis, causative agent of the disease called apple scab, is the trait that was introduced. No further marker or other genes are present.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The scab resistance gene, HcrVf2, derived from apple itself, under control of its own promoter (cisgenic lines) or under control of the apple rubisco promoter, (intragenic line)has been inserted. No marker genes or other genes are present; one non-transcribed, non-functional element, i.e. a Recombination site (Rs) derived from Zygosaccharomyces rouxii is left behind after recombination-based marker excision producing marker-free plants

6. Brief description of the method used for the genetic modification:
Agrobacterium-mediated transformation of young, in vitro apple leaf explants.

Experimental Release

1. Purpose of the release:
Testing in a field situation, comparable to a production apple orchard, the resistance against apple scab of cis- and intragenic apple cv. Gala lines, carrying the HcrVf2 resistance gene, that is also present in conventionally bred, resistant cultivars, such as Santana, Topas and Florina.

2. Geographical location of the site:
Municipality of Wageningen, The Netherlands

3. Size of the site (m2):
1750 m2

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
No specific environmental impact is expected. The trait and the gene that is introduced are already present within the apple germplasm. Benchmarks are provided by the resistant cultivars carrying the same gene originating from conventional breeding programs. Such non-GM apple cultivars with this particular gene are being cultivated and commercialized for approximately 12 years now without any known positive or negative effects on the environment other than that less chemoprotectants have to be used in cultivation. Therefore, no direct or indirect selective advantages in natural environments are to be expected for the GMPts carrying this gene; cultivation of elite, high quality apple cultivars equipped with this gene by GM will contribute to the desired reduction in the use of chemicals. This can be regarded as a benefit for growers and consumers.

Brief description of any measures taken for the management of risks:
Control of risks is achieved by the small scale of the trial, by the use of a gene derived from apple itself. Apples will not enter the food chain and will be collected, scored for symptom development and destroyed. An isolation distance of 150 metres will be implemented within which no growth or cultivation of other apples will take place. No pollinators will be used, only hand pollination. Monitoring will take place with a minimum of four times per month during the growing season.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
A secondary goal of this field trial is to gain data on environmental and other aspects required by the Dutch government to be able to prepare for so-called Category 2 field trials with these cisgenic and/or intragenic apple lines.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known