General informationNotification NumberB/NL/07/04Member State to which the notification was sentNetherlandsDate of acknowledgement from the Member State Competent Authority11/06/2007Title of the ProjectPotato plants with reduced amylose contentProposed period of release:01/01/2008 to 31/12/2015Name of the Institute(s) or Company(ies)AVEBE, ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:The GM-potato is characterized by a high level of amylopectin and a low level of, or no amylose. No selection marker genes are used for transformation.Genetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:- T-DNA borders from Agrobacterium tumefaciens for stable incorporation into the plant genome.
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
- Granule-bound starch synthase (GBSS) cDNA, from potato, in sense and antisense orientation for downregulating GBSS expression.
- GBSS promoter from potato for regulating gene expression.
- GBSS terminator from potato or Nopaline synthase gene terminator from Agrobacterium tumefaciens for regulation of gene expression.6. Brief description of the method used for the genetic modification:T-DNA was introduced into potato varieties by Agrobacterium-mediated gene transfer technology using a binary vector system.7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:T-DNA was introduced into potato varieties by Agrobacterium-mediated gene transfer technology using a binary vector system.Experimental Release1. Purpose of the release:- investigating the agricultural value of modified lines.
- evaluating the starch composition under field conditions.
- producing seed potatoes for future trials2. Geographical location of the site:Municipalities: Borger-Odoorn, Pekela, Veendam, Emmen, Eemsmond, AA en Hunze, Coevorden, De Marne, in The Netherlands.3. Size of the site (m2):14 sites of 5,000 m2 and 3 sites of 15,000 m2 with a maximum of 100,000 m2/year4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:There have been previous releasers of potato lines transformed with the same or similar constructs in the Netherlands. No adverse impact of the environment or human health have been recorded in any of the trials.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The introduced trait, reduction of amylose content, will not directly or indirectly confer an increased selective advantage in natural environments.Brief description of any measures taken for the management of risks:All handling of GMO fields will bedone according to Good Agricultural Practices.
- transportation of potatoes will be done in bags in open containers.
- crop rotation: no potato crop the following two years.
- destruction of volunteers.
- destruction of waste material.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:Yes10/01/2008 00:00:00Remarks: