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Notification report


General information

Notification Number
B/NL/04/04

Member State to which the notification was sent
Netherlands

Date of acknowledgement from the Member State Competent Authority
03/09/2004

Title of the Project
Multiplication and field testing of transgenic potatoes altered in their carbohydrate metabolism, resulting in amylose-free tubers.

Proposed period of release:
01/01/2004 to 31/12/2013

Name of the Institute(s) or Company(ies)
AVEBE, ;


3. Is the same GMPt release planned elsewhere in the Community?
Not known

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/NL/03/04;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumall cultivars

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Potato with an increased starch production (anti-GBSS) without any selection markers.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
pKGBA50mf-IR1.1 contains between the borders:
KGZ-prom: (=GBSS) Tuber specific and sugar inducible promoter of the KGZ gene from Solanum tuberosum;
1.1 kb of sense DNA sequences of the KGZ gene from Solanum tuberosum encoding the N-terminus of the KGZ protein;
AS-KGZ: anti-sense strand of the KGZ gene from Solanum tuberosum.

pIRMASmf contains between the borders:
KGZ-prom: (=GBSS) Tuber specific and sugar inducible promoter of the KGZ gene from Solanum tuberosum;
M-GBSSI (antisense): 617 bp (bps. 1070-1687) of antisense strand of KGZ cDNA from Solanum tuberosum;
M-GBSSI (sense): 761 bp (bps. 926-1687) of KGZ-cDNA from Solanum tuberosum.


6. Brief description of the method used for the genetic modification:
The genetic material is inserted by Agrobacterium tumefaciens cq Agrobacterium rhizogenes mediated transformation. Prior to the introduction on the fields the studied plant lines will be checked for the absence of vector backbone sequences by PCR. Only vector-free plants will be introduced in the field.

Experimental Release

1. Purpose of the release:
Field testing and propagation of genetically modified potatoes.

2. Geographical location of the site:
North-East part of the Netherlands

3. Size of the site (m2):
1) Maximum of 10 sites per year.
2) Maximum total area per year of 5 hectares per year.


4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The same GM-plant is part of the B/NL/01/11 and B/NL/03/04. No special potential environmental and human health impacts are expected nor detected.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Similar plants with the same new trait have been evaluated extensively in previous field experiments. The experiments did not reveal an altered sensitivity for frost for both the genetically modifiied plants and the amylose-free mutants. It is therefore not likely that the genetic modification will result in an altered frost sensitivity.

The GMP's do not contain any other introduced gene. The potential impact will therefore be limited to the trait of an altered carbohydrate composition. Other potential impacts on the environment are therefore considered to be extreme unlikely.


Brief description of any measures taken for the management of risks:
Besides the inactivation of the waste, no specific measures are envisaged. Volunteer plants will be removed in accordance with good agricultural practice.

Before the genetically modified potato plants are released in the environment, they will be checked for the absence of vector backbone sequences. Only those plants without vector backbone sequences will be used in the actual field trials.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
No effects are expected. The monitoring of the plants, as also in traditional breeding, is to observe deviations compared to the parent cultivars. In an area , the walks and first row of non-GMO potatoes around the GMO field, the volunteers will be observed.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
25/01/2005 00:00:00
Remarks: