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Notification report


General information

Notification Number
B/LT/06/01

Member State to which the notification was sent
Lithuania

Date of acknowledgement from the Member State Competent Authority
26/10/2006

Title of the Project
Notification for the field testing of spring oilseed rape (Brassica napus) genetically modified for improved oil composition in the seed

Proposed period of release:
01/04/2007 to 31/10/2011

Name of the Institute(s) or Company(ies)
BASF Plant Science GmbH, Carl-Bosch-Strasse 38, D-67056 Ludwigshaven GERMANY;


3. Is the same GMPt release planned elsewhere in the Community?
Yes:
Sweden;

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):


Other notifications
Chile

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
canolabrassicaceaebrassicabrassica napusnapus (syn. oleifera)Westar

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- improved oil composition in the seed
- nptII gene (neomycin phosphotransferase II) as selectable marker gene to identify transgenic cells in tissue culture


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
napin promoter from Brassica napus
Delta-6 desaturase gene from Phytium irregulare
Delta-5 desaturase gene from Thraustochytrium spp.
Delta-6 elongase gene from Physcomitrella patens
Delta-12 desaturase gene from Calendula officinalis
ocs (octopin synthase) terminator from A. tumefaciens
recombination sites mr-attB1 and mr-attB2 used for construction of the vector
Promoter and terminator from nopaline synthase (nos) gene, from A. tumefaciens,
nptII gene (neomycin phosphotransferase II) from E. coli


6. Brief description of the method used for the genetic modification:
Plasmid DNA was introduced into the rape seed lines by Agrobacterium-mediated gene transfer technology. This is standard technology for Brassica napus transformation.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
The specific purpose of the release is the evaluation of the performance of the added trait under field conditions for research purposes.

2. Geographical location of the site:
The 2 release sites will be located in the Klaipeda municipality.

3. Size of the site (m2):
The genetically modified plants will occupy no more than 1 ha (10,000m2) in an experimental plot of less than 2 ha.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
No previous releases have been carried out.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified oilseed rape lines show an altered fatty acid composition in seeds towards very long-chain polyunsaturated fatty acids. The plant material also shows tolerance to the aminoglycoside type antibiotics kanamycin and neomycin in tissue culture.
The added traits are not likely to have a critical influence under field conditions on any important competitive factors such as pollen production/dispersal, fertility or compatibility, nectar production, seed dispersal or general fitness. Based on observations in the greenhouse the genetically modified oilseed rape lines do not differ from the parental control lines in their morphology, flowering time, germination rate or kernel weight. No difference regarding persistence in agricultural habitats or invasiveness in natural habitats or persistence in agricultural habitats compared to conventional oilseed rape is expected.
Considering the very low likelihood of the introduced traits to confer increased fitness or the GM oilseed rape lines in any other way to become more persistent than the parental Brassica napus plant material in agricultural habitats or more invasive in natural habitats, the overall impact is expected to be negligible.
Any effects from the nptII gene on non-target organisms are not expected and have not been reported from other field trials harbouring this trait. The nptII gene is already present in the environment, since it is derived from soil organisms.


Brief description of any measures taken for the management of risks:
An isolation distance of 500m to other Brassica crops will be observed. Planting and harvesting equipment will be cleaned on site to prevent the dispersal of GM seeds. There will be no oilseed rape or any other crossable crop cultivation on the release area the following four years after the release. Potential volunteers will be monitored and removed according to conventional agricultural practice. During the release the trial site will be monitored at defined intervals.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
No
06/04/2007 00:00:00
Remarks: