Member State to which the notification was sent
Date of acknowledgement from the Member State Competent Authority
Title of the Project
Open field evaluation of transgenic wild strawberry (Fragaria x ananassa Duch.) with the chimeric defH9-iaaM gene
Proposed period of release:
01/01/2003 to 31/12/2006
Name of the Institute(s) or Company(ies)
UNIVERSITA' ANCONA - Facoltà di agraria -Dipartimento di biotecnologie agrarie e ambientali, ;
3. Is the same GMPt release planned elsewhere in the Community?
Has the same GMPt been notified elsewhere by the same notifier?
Genetically modified plant
Complete name of the recipient or parental plant(s)
strawberry (adj. ananassa)
fragaria fragaria x fragaria ananassa
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Two genes were inserted into the genomic DNA which encode for two proteins with different function:
1) the enzyme tryptophan-2-monooxygenase which confers parthenocarpic fruit development;
2) the enzyme neomycin acetyl-phosphotransferase II which confers resistance to the antibiotics neomycin and kanamycin;
Thus the GMP display the following traits:
1. in several type of fruits the expression of the chimeric DefH9-iaaM gene showed ability to set and develop normal seedless fruits under unfavourable environmental condition (T°, RU, light) for pollination and/or fertilization which prevent the normal development of carpel to fruit in untransformed. In strawberry will be evaluated mainly the effect on fruit size, firmness and quality;
2. kanamycin resistance, determined either as the ability of plants to grow on kanamycin containing medium or to detoxify the antibiotic after spraying it on leaves of the young plant which do not show any chlorotic symptom.
The GMP do not show any other phenotypical difference compared to untransformed wild type strawberry.
3. Type of genetic modification:
In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The inserted DNA region corresponds to the T-DNA of Agrobacterium plasmid pCarp-iaaM which includes two physically linked expression cassettes:
- a 5.5 Kb chimeric gene, conferring parthenocarpic fruit development, obtained by a transcriptional fusion of the DefH9 promoter from Anthirrinum majus which allows specify expression in the ovules during flower and fruit development. The DefH9 promoter drives the expression of the codin region of the iaaM gene from Pseudomonas syringae pv.savastonoi which codifies for the enzyme triptophan-2-monooxygenase involved in the synthesis of the IAA auxin..
- A 1.5 Kb chimeric gene, conferring kanamycin resistance phenotype, obtained by the insertion of the neomycin phosphotransferase (NPTII) gene from Escherichia coli which codifies for the detoxifying enzyme, between the NOS promoter and the NOS terminator from the nopaline synthetase gene of Agrobacterium tumefaciens. It is the selectable marker employed to select transformants.
6. Brief description of the method used for the genetic modification:
Genetic transformation was achieved by in vitro infection of leaf, cotyledon and hypocotyls explants with the disarmed strain GV3101 of Agrobacterium tumefaciens carrying the binary plasmid pCarp-Ri-iaaM which harbours the chimeric T-DNA. Transgenic plants were selected in kanamycin containing medium.
1. Purpose of the release:
To verify under open field cultivation condition the agronomical and technological characteristics of transgenic clones; especially to verify the effect of the pCarp-iaaM construct on cultivated fruit set, development and quality
2. Geographical location of the site:
Agugliano (Ancona) Italy
3. Size of the site (m2):
Field trial: 200 m2 for each of the three production cycles
Environmental Impact and Risk Management
Summary of the potential environmental impact from the release of the GMPts:
In the sites where trials will be carried out, potential gene transfer from GMPs is possible only to other strawberries. This will be avoided by setting up the trials within an area free from commercial strawberry crops.
Furthemore no genetic compatibility is possible within the wild strawberry know for our country (F.vesca) and for any other species
Brief description of any measures taken for the management of risks:
No uncontrollable spreading of GMP genetic material is anticipated.
- Field will be set in an area isolated from strawberry crops and identified by the notification number;
- During the blossom period the trail will be protected to avoid pollen dispersion in the surrounding environment;
- All fruits will be collected, boiled to inactivate tissue and seeds, and stored;
At completion of the trial, the plants will be destroyed by using systemic herbicide active on strawberry. The destroyed plants and the fruits will be incorporated into the soil, the area will be scouted to eliminate eventual volunteer strawberry plants.
European Commission administrative information
Consent given by the Member State Competent Authority: