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Notification report


General information

Notification Number
B/HU/08/3

Member State to which the notification was sent
Hungary

Date of acknowledgement from the Member State Competent Authority
04/02/2008

Title of the Project
Field testing of genetically modified wheat (Triticum aestivum L.) lines for improved red rust resistance (functional genomic research)

Proposed period of release:
03/03/2008 to 03/03/2012

Name of the Institute(s) or Company(ies)
Department of Biotechnology, Cereal Research Non-profit Company, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
wheatpoaceaetriticumtriticum aestivumLaboratory used wheat lines

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The genes used for transformation in order to improve red rust resistance were isolated from:
Triticum monococcum L. (NBS-LRR genes);
Trichoderma hamatum (thamchi1);
Coniothyrium minitans (fungus) (cmg1).
The marker gene is the ‘bar’ gene originating from Streptomyces hygroscopicus bearing bialaphos or ppt resistance (bialaphos selection)


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
NBS-LRR genes have been isolated from Triticum and it was used for Triticum transformation (cisgenic transformation).
The 1.4301 bp long ‘thamchi1’ gene has been isolated from Trichoderma hamatum.
The 2.336 bp long ‘cmg1’ gene has been isolated from Coniothyrium minitans.
The maize ubiquitin-1’ promoter is 2.073 bp long and originates from maize.
The ‘nos’ terminator region is 329 bp long and originates from Agrobacterium tumefaciens.


6. Brief description of the method used for the genetic modification:
The vector DNA – which carries the transgenes – is delivered into the wheat cells by a direct method called genetic bombardment (device PDS 100/He).

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
no applicable

Experimental Release

1. Purpose of the release:
The main goal of the experiments is functional genomic research. The functions of the targeted genes will be studied. Further purpose of the release is to determine the effect of the transformed genes for wheat red rust resistance.

2. Geographical location of the site:
The experiments have been designed at “Kecskés” Experimental Station of Cereal Research Non-profit Co., in Szeged (Southern part of Hungary) - Szeged III. kerület 02242

3. Size of the site (m2):
maximum 5000 m2 (including non-transgenic controls and safety border rows)

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The bar marker gene is included in different transgenic crops. There are no negative data regarding human health. The genes – theoretically bearing red rust resistance to the recipient - under the tests are not tested yet in safety studies. These experiments are motivated by functional genomic research only. The GM plants from these experiments will not enter the food or feed chain.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The wheat is strictly self-pollinated. The risk for out-crossing is close to the zero. The experiments have been designed under strict safety conditions controlled by the Hungarian authority. All of the seed material (excluding the experimental sample) will be destroyed (burned).

Brief description of any measures taken for the management of risks:
2 meter isolation distance as well as 2 meter border rows will be sown. In the growing season the authority will control the application of all prescribed safety measures. A dicotyledonous crop plant (sweet pepper, pea, rapeseed, mustard etc.) will be sown after the experiment where the crop management would eliminate all remained transgenic plants (from the previous generation). Field preparation for follow up sowing (plough, harrow) serves this aim too. The experiment would be surrounded by buffer zones in order to prevent pollen from spreading by a physiological and biological shield. Wheat will be harvested mostly by hand and with combine if the parcel is bigger. The combine will be cleaned before and after harvesting, and harvested grains will be sacked separately and stored in a closed room. A net will be used in order to prevent the wheat field from birds. Grains which are not processed for further experiments will be destroyed by burning (including grains of buffer zones).

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Transgenic wheat will be used only for experimental purposes. If the experiments will produce positive resistance data, we will start risk assessments.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known