General informationNotification NumberB/HU/07/06Member State to which the notification was sentHungaryDate of acknowledgement from the Member State Competent Authority12/04/2007Title of the ProjectField trials with drought tolerant potato varieties expressing the trehalose-6-phosphate synthase gene of yeast.Proposed period of release:01/04/2007 to 30/09/2009Name of the Institute(s) or Company(ies)Agricultural Biotechnology Center, Agricultural Biotechnology Center, Gödöllő;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:Drought tolerant TPS1 transgenic line of potato cultivar White LadyGenetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:The donor organism was the bakers’ yeast (Saccharomyces cerevisiae). Elements of the insert region are:
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
|potato||solanaceae||solanum||solanum tuberosum||tuberosum||White Lady|
- Right border of the Ti plasmid of Agrobacterium to initiate DNA transfer.
- 1140-bp promoter region of the drought-inducible gene DS2 derived from the potato cultivar White Lady (accession number: AJ320154) to provide expression for the TPS1 structural gene encoding the enzyme trehalose-6-phosphate synthase.
- The TPS1 structural gene of yeast encoding the enzyme trehalose-6-phosphate synthase (accession number: X68214) to improve drought tolerance of potato.
- The terminator region of the gene nos to terminate transcription of TPS1.
- Left border of the Ti plasmid of Agrobacterium to terminate DNA transfer.6. Brief description of the method used for the genetic modification:Agrobacterium-mediated transformation of sterile potato tuber slices (cultivar White Lady) according to the Hungarian Patent No. 221 514.7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:Not applicable.Experimental Release1. Purpose of the release:The purpose of the release is to testing the drought tolerance and yield of the TPS1 transgenic potato line under field conditions.2. Geographical location of the site:The release site will be the inner and outer breeding area of the Potato Research Center of the Agricultural Faculty of Pannon University. The inner breeding area is approximately 0.5 hectares and situated in Keszthely city (in the non-populated area), near the research buildings of the Center. The outer breeding area, which is 10 hectares in space, is located in a distance of 300 meter from the research buildings and 300-500 meter from the Keszthely-Szentendre yard.3. Size of the site (m2):In the first year 100 m2 will be used, later at 300 m2.4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:It was not released formerly.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:Under the climatic and geographical conditions of Hungary, potato is almost an exclusively self-pollinating species. In Europe, and thus in Hungary, there are no wild or cultivated plant species capable of cross-pollination with potato. The cross-pollinating relatives of potato (for example S. demissum, S. vernei, etc.) live in Middle- and South-America.
No dangerous effects were identified.Brief description of any measures taken for the management of risks:Under the climatic and geographical condition of Hungary, potato is almost exclusively self-pollinated. This was supported by own decades of experience. We have found that male-sterile plants grown side by side with fertile genotypes were only very rarely fertilized. Not even in a single case spreading of kanamycin resistance gene to 5 m circle to the other plants was detected. The plant is self-steril and the seeds can not overwinter and spread. Spreading of the transgene by pollens is not possible as it is described above. The only way of spreading of potato in cultivated areas is the growth of plants from overwintered tubers. The genetically modified potato line that we have isolated can be identified by morphological markers and PCR technique.
Chemical and mechanical defoliation before harvest. Elimination of rest of the tubers depending on the amount by vapour sterilisation or digging in ground. To prevent survival of tubers in winter shallow cultivation for freezing and chemical weed control can be applied.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:Not applicable to this release.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:Not known