Notification report

General information

Notification Number

Member State to which the notification was sent

Date of acknowledgement from the Member State Competent Authority

Title of the Project
Field trials program of genetically modified maize varieties resistant to certain Lepidopteran and Coleopteran insects and tolerant to glufosinate-ammonium herbicide (1507x59122 maize)

Proposed period of release:
01/04/2005 to 31/12/2008

Name of the Institute(s) or Company(ies)
Pioneer Hi-Bred Hungary Ltd., ;

3. Is the same GMPt release planned elsewhere in the Community?

Has the same GMPt been notified elsewhere by the same notifier?

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
maizepoaceaezeazea maysmaysexperimental varieties

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The genetically modified 1507x59122 maize varieties are resistant to certain Lepidopteran insects, such as the European corn borer (Ostrinia nubilalis) and to certain Coleopteran insects, such as the Western corn rootworm (Diabrotica virgifera virgifera) and can tolerate the use of glufosinate-ammonium herbicide for the weeding of maize field. This maize was derived through traditional breeding methods between progeny of genetically modified 1507 maize, which is resistant to certain lepidopteran insects and tolerant to glufosinate herbicide, and 59122 maize, which is resistant to certain coleopteran insects and tolerant to glufosinate herbicide.

Genetic modification

3. Type of genetic modification:

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
1507x59122 maize contains the following genetic elements:
- the cry1F gene from Bacillus thuringiensis var. aizawai that confers resistance to certain Lepidopteran insects, such as Ostrinia nubilalis, and the pat gene from Streptomyces viridochromogenes that confers tolerance to glufosinate-ammonium herbicide, from 1507 maize
- the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis strain PS149B1 that confer resistance to certain Coleopteran insects, such as Diabrotica virgifera virgifera, and the pat gene from Streptomyces viridochromogenes that confers tolerance to glufosinate-ammonium herbicide, from 59122 maize
All these genes have been introduced with only their regulatory components necessary for expression in maize plant.

6. Brief description of the method used for the genetic modification:
No genetic modification was made on the 1507x59122 maize directly: it was derived through traditional breeding methods between progeny of the genetically modified 1507 and 59122 maize.
1507 maize was genetically modified using a particle acceleration method, 59122 maize was genetically modified using Agrobacterium transformation method.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
Several types of trials will be carried out, such as trials for protein expression analyses, effects on non-target organisms, agronomic value and insect resistance, and other trials in view of registration on the national List of Hungary.

2. Geographical location of the site:
In 2005, the location of the release site will be as follows:

County Fejér:
- Enying, Agrár Rt. , Kiscséri puszta, B-2 tábla
- Füle, Búzakalász Mezőgazdasági Szövetkezet, F-14 tábla

County Pest:
- Sóskút, Sóskút Fruct Gyümölcstermelő Kft., hrsz. 0106

3. Size of the site (m2):
The surface of genetically modified maize plants that will be planted at each location in 2005 will be up to 4000 m². The total surface area taken up by the trials (all varieties and borders included) being higher.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Field trials have already been conducted at different locations across maize growing regions in North America, South America and Europe, and no environmental problems were reported for these trials. The transgenic maize plants were indistinguishable from the non-genetically modified maize plants except for showing resistance to certain Lepidopteran insects, such as European corn borer, and to certain Coleopteran insects, such as Western corn rootworm, and showing tolerance when glufosinate herbicide was applied, traits due to the genetic modifications.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from the deliberate release of genetically modified maize resistant to certain Lepidopteran and Coleopteran insects and tolerant to glufosinate herbicide are expected, as per information contained in the environmental risk assessment included in the notification.

Brief description of any measures taken for the management of risks:
The shedding of pollen from the genetically modified plants will be controlled by maintaining a 200-meter isolation distance with any other non-experimental maize crop. In addition, the trial site will be surrounded by four border rows of conventional maize of a similar relative maturity that will be also destroyed at the end of the release.
Seed dispersal of individual kernels does not generally occur. They are fixed on a cob and enclosed in many husks that protect the seeds from outside contact. When a few seeds need to be collected for analysis; this will be done by sampling the whole ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by application of a non-selective herbicide other than glufosinate or by mechanical destruction, and incorporation into the soil.
At the end of the release, all remaining plant matter that has not been harvested for analyses will be destroyed by chopping and incorporating into the soil.
Volunteer maize will be monitored during the following year. An herbicide treatment (other than glufosinate) will be used in order to ensure destruction if needed.
There will be no commercial maize grown on te same field the following year.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Trials for effects on Non-Target Organisms
The purpose of the release is to evaluate the impact of the cultivation of insect-resistant 1507x59122 maize hybrids on key non-target arthropod populations, in comparison with the cultivation of non-transgenic equivalent maize hybrids treated with chemical insecticide and untreated.

Final report

European Commission administrative information

Consent given by the Member State Competent Authority:
Not known