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Notification report


General information

Notification Number
B/GB/18/01

Member State to which the notification was sent
United Kingdom

Date of acknowledgement from the Member State Competent Authority
20/02/2018

Title of the Project
Genetic improvement of Camelina seeds

Proposed period of release:
01/05/2018 to 30/09/2020

Name of the Institute(s) or Company(ies)
Rothamsted Research, West Common, Harpenden
Hertfordshire,
AL5 2JQ
UK;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/GB/16/R8/01;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
Gold-of-pleasure, false flaxBrassicaceaeCamelinasativaCeline

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
 

Construct ID

Phenotype

# of genes

Marker

1

DHA2015.1

Omega-3 LC PUFAs seed oil

Seven (1)

DsRed

2

DHA2015.2

Omega-3 LC PUFAs seed oil

Six (1)

DsRed

3

DHA2015.3

Omega-3 LC PUFAs seed oil

Six (1)

DsRed

4

DHA2015.4

Omega-3 LC PUFAs seed oil

Six (1)

DsRed

5

DHA2015.5

Omega-3 LC PUFAs seed oil

Eight (1)

DsRed

6

EPA2015.4

Omega-3 LC PUFAs seed oil

Four (1)

DsRed

7

EPA2015.8

Omega-3 LC PUFAs seed oil

Five (1)

DsRed

8

EPA2016.1

Omega-3 LC PUFAs seed oil

Four (1)

DsRed + BAR

9

ASX-A2

Astaxanthin in seeds

Three (1)

BAR

10

THIO14

Medium chain fatty acids in seeds

One (1)

DsRed

11

MaMa14-6*

Wax esters in seeds

Three (1)

DsRed

12

GDH

Enhanced photosynthesis

One (1)

DsRed

13

GDH-PP

Enhanced photosynthesis

One (1)

DsRed

14

MAP22

Improved architecture

One (2)

DsRed

         

* = two events from this construct will be tested

1 = synthetic, codon-optimised sequence

2= native sequence



Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:

Construct DHA2015.1

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Phytophora infestans

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

PsD12

1197bp

Synthetic

Encodes a fatty acid D12-desaturase FAD2 activity from Phytophora sojae

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

OtElo5

903bp

Synthetic

Encodes an acyl-CoA dependent D5-elongase from the marine picoalgae Ostreococcus tauri

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

O809D4

1480bp

Synthetic

Encodes a fatty acid D4-desaturase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

Construct DHA2015.2

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

OtElo5

903bp

Synthetic

Encodes an acyl-CoA dependent D5-elongase from the marine picoalgae Ostreococcus tauri

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

O809D4

1480bp

Synthetic

Encodes a fatty acid D4-desaturase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

DHA2015.3

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

OtElo5

906bp

Synthetic

Encodes an acyl-CoA dependent D5-elongase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

O809D4

1480bp

Synthetic

Encodes a fatty acid D4-desaturase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

DHA2015.4

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

OtElo5

906bp

Synthetic

Encodes an acyl-CoA dependent D5-elongase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

O809D4

1653bp

Synthetic

Encodes a fatty acid D4-desaturase from the marine diatom Thalassiosira pseudonana

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

DHA2015.5

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Phytophora infestans

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

PvArc

1138bp

Phaseolus vulgaris

Arcelin-5 seed specific promoter

PerfD15

1176bp

Perilla frutescens

Encodes a fatty acid D15-desaturase FAD3 activity from Perilla frutescens

PvArct

600bp

Phaseolus vulgaris

Arcelin-5 gene terminator

NP

664bp

Brassica napus

Napin seed specific promoter

PsD12

1197bp

Synthetic

Encodes a fatty acid D12-desaturase FAD2 activity from Phytophora sojae

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

OtElo5

903bp

Synthetic

Encodes an acyl-CoA dependent D5-elongase from the marine picoalgae Ostreococcus tauri

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

O809D4

1480bp

Synthetic

Encodes a fatty acid D4-desaturase from the marine picoalgae Ostreococcus RCC809

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

EPA2015.4

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Emiliana huxleyi

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Mantionella squamata

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

EPA2015.8

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Ostreococcus tauri

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

PvArc

1138bp

Phaseolus vulgaris

Arcelin-5 seed specific promoter

PerfD15

1176bp

Perilla frutescens

Encodes a fatty acid D15-desaturase FAD3 activity from Perilla frutescens

PvArct

600bp

Phaseolus vulgaris

Arcelin-5 gene terminator

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

EPA2016.1

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

pNOS

288bp

Agrobacterium tumefaciens

Nopaline synthase gene promoter

bar

548bp

Synthetic

Encodes phosphinothricin acetyl transferase

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

USP

684bp

Vicia faba

Unknown Seed Protein Seed-specific promoter

PSE1

873bp

Synthetic

Encodes an acyl-CoA-dependent D6-elongase from the moss Physcomitrella patens

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

TcD5

1320bp

Synthetic

Encodes a fatty acid D5-desaturase from the marine species Thraustochytrium

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

SBP

1800bp

Arabidopsis thaliana

Sucrose-binding protein promoter (seed-specific)

OtD6

1665bp

Synthetic

Encodes a fatty acid D6-desaturase from the marine picoalga Mantionella squamata

CatpAt

235bp

Arabidopsis thaliana

Cathepsin A gene terminator sequence

NP

664bp

Brassica napus

Napin seed specific promoter

Piw3

1086bp

Synthetic

Encodes a fatty acid w3-desaturase from Hyaloperonospora parasitica

E9t

558bp

Arabidopsis thaliana

Ubiquitin E9 ligase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

ASX-A2

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

GLY

702bp

Glycine max

11S Seed storage protein (Glycinin) promoter

HBFD1

1226bp

Synthetic

Encodes carotenoid 4-hydroxy-β-ring 4-dehydrogenase from Adonis aestivalis

Glyt

451bp

Glycine max

11S storage protein (Glycinin) terminator

GLY

702bp

Glycine max

11S Seed storage protein (Glycinin) promoter

ZmPhys

1232bp

Synthetic

Encodes phytoene synthase from maize

pNOS

288bp

Agrobacterium tumefaciens

Nopaline synthase gene promoter

bar

548bp

Synthetic

Encodes phosphinothricin acetyl transferase

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

GLY

702bp

Glycine max

11S Seed storage protein (Glycinin) promoter

CBFD2

687bp

Synthetic

Encodes carotenoid β-ring 4-dehydrogenase from Adonis aestivalis

Glyt

451bp

Glycine max

11S storage protein (Glycinin) terminator

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

THIO14

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

CpTHIO14

1270bp

Synthetic

Encodes a FATB2 thioesterase from Cuphea palustris

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

MaMa14-6

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

CpTHIO14

1270bp

Synthetic

Encodes a FATB2 thioesterase from Cuphea palustris

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

MaFAR

1539bp

Synthetic

Encodes a fatty acyl-CoA reductase form Marinobacter aquaeolei

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CNL

1064bp

Linum usitatissimum

2S seed storage protein (Conlinin) promoter

MhWS

1427bp

Synthetic

Encodes a wax synthase from Marinobacter hydrocarbonoclasticus

OCSt

192bp

Agrobacterium tumefaciens

octopine synthase gene terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

MAP22

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

p35S

446bp

Cauliflower mosaivc virus

35S promoter

MAP22

1455bp

cDNA

Encodes a microtubule associated protein with a calmodulin binding domain from Arabidopsis thaliana

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

GDH

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

p35S

446bp

Cauliflower mosaivc virus

35S promoter

ChlTP

240bp

Synthetic

Encodes cleavable plastid targeting sequence derived from the Arabidopsis thaliana SSU-Rubisco

GlcD,E,F

3768bp

cDNA

Encodes Subunits D, E & F of the Glycolate Dehydrogenase from Escherichia coli

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border

GDH-PP

Element

Size

Donor Organism

Description and Intended Function

RB

24bp

Agrobacterium tumefaciens

T-DNA Right border

p35S

446bp

Cauliflower mosaivc virus

35S promoter

ChlTP

240bp

Synthetic

Encodes cleavable plastid targeting sequence derived from the Arabidopsis thaliana SSU-Rubisco

GlcD,E,F

3888bp

cDNA

Encodes Subunits D, E & F of the Glycolate Dehydrogenase from Escherichia coli with self-processing A2 linkers

35St

216bp

Cauliflower mosaic virus

35S transcript terminator sequence

CsVMV

528bp

Cassava vein mosaic virus

Cassava vein mosaic virus (CsVMV) promoter

DsRed

684bp

Synthetic

Encodes a florescent protein from Discosoma spp.

NOSt

256bp

Agrobacterium tumefaciens

Nopaline synthase gene terminator sequence

LB

23bp

Agrobacterium tumefaciens

T-DNA Left border



6. Brief description of the method used for the genetic modification:
Plasmid DNA was inserted into the C. sativa genome using Agrobacterium-mediated floral transformation system and transgenic plants were identified on the basis of bialaphos resistance or visual identification (DsRed expression)

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
n/a

Experimental Release

1. Purpose of the release:
The purpose of this trial is to learn more about the “real world” agronomic performance of GM camelina plants that have been modified with the capacity to synthesize non-native lipids such as omega-3 long chain polyunsaturated fatty acids, ketocarotenoids and wax esters. It will also evaluate the performance of additional traits which might enhance field performance, such as altered plant architecture or enhanced photosynthesis.

2. Geographical location of the site:
It will be sited on the experimental farm at Rothamsted Research, Harpenden, at grid reference TL120130, and also at the experimental farm at Rothamsted Research, Brooms Barn, at grid reference TL756654.

3. Size of the site (m2):
The total area of GM plants per year will maximally be 4,306m2

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The majority of the lines have not previously been released. Two lines (DHA2015.1, ASX-A2) have previously been released and whilst no data has yet been published on these releases, no issues were observed during the trials

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
Of the fourteen different GM C. sativa lines listed above in (2), eleven are indistinguishable from the non-GM equivalent except for the modified composition of their seeds, in particular by the presence of the health-beneficial omega-3 long chain polyunsaturated fatty acids EPA and DHA and/or the ketocarotenoid astaxanthin, or wax esters. This modified composition is found only in the seeds of the GM C. sativa and is absent from all other vegetative tissues (e.g. leaves, roots, stems). In the other three cases, the transgenes are expressed in all tissues, but neither they nor their products nor any resulting metabolites are known or expected to be harmful in anyway.

The gene donor organisms are not known to be pathogenic or allergenic to humans, and none of the genes under investigation or the selectable or visual marker genes, are expected to result in the synthesis of products that are harmful to humans, other organisms or the environment. Any unknown hazards arising from the expression and ingestion of foreign proteins will not occur since the C. sativa plants will not be consumed by humans.

The probability of C. sativa seeds escaping from the trial site or the transfer of inserted characteristics to sexually-compatible species outside the trial area is estimated as very low. C. sativa seeds are moderate in size and not normally dispersed by wind. Management measures including the use of gas guns and hawk kites will be employed to mitigate the risk of seed removal by birds. Management procedures to minimise the spread of seeds or pollen will further reduce the probability of these events occurring. There will be no compatible species grown for 1000 meters from the boundary of the site and no sexually-compatible wild relatives of C. sativa exist in the vicinity of the Rothamsted farm.

The risk of non-sexual, horizontal gene transfer to other species is extremely low. In the event of horizontal gene transfer to bacteria, neither the trait genes nor the marker genes would be expected to confer a selective advantage in the field environment under consideration. The area proposed to be planted with GMOs is small and temporary (lasting between 4 and 5 months).

Bearing in mind its limited scope, overall risk of harm to human health or the environmental arising from this trial is assessed as very low.


Brief description of any measures taken for the management of risks:
No C. sativa or related species will be grown within 1000m from the trial. The trial plots will be surrounded by a pollen barrier of non-GM camelina.


The release site will be visited by trained laboratory personnel who are working on the project at no less than weekly intervals (and at some periods, daily) during the growing season of each year of the trial. Any unexpected occurrences that could potentially result in adverse environmental effects or the possibility of adverse effects on human health will be notified to the Defra immediately. Should the need arise to terminate the release at any point the emergency plans detailed below will be followed.

At the end of each season, the plot will remain in stubble and monitored for volunteers during the remainder of the year and the following season. Any volunteers identified will be destroyed by herbicide treatment (e.g. glyphosate) or removed by hand and destroyed.

Following completion of the two-year trial the release site will remain fallow for a further season to enable easy identification of volunteers. The site will be inspected regularly and any volunteers identified will be immediately destroyed either by application of a systematic broad leaf herbicide.

In the unlikely event that the integrity of the site is seriously compromised, the trial will be terminated and all plants, (including GM and control C. sativa plots, and cereal separator) will be destroyed using a suitable herbicide or harvesting as deemed appropriate. All harvested material will be removed from the site and disposed of by incineration or deep burial at a local authority-approved landfill site using an approved contractor. Transportation of waste materials will be in secure containers. The phone numbers of all key staff will be available to site security and farm.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
n/a

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
17/05/2018 00:00:00
Remarks: