Member State to which the notification was sent
Date of acknowledgement from the Member State Competent Authority
Title of the Project
Field experimentation of corn genetically modified for flowering earliness
Proposed period of release:
01/04/2007 to 30/11/2010
Name of the Institute(s) or Company(ies)
3. Is the same GMPt release planned elsewhere in the Community?
Has the same GMPt been notified elsewhere by the same notifier?
Genetically modified plant
Complete name of the recipient or parental plant(s)
A188 line and experimental hybrids
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Modification of flowering earliness
Transgenic plants contain the bar gene conferring resistance to glufosinate, most of the transformation events contain the Green Fluorescent Protein (GFP) as a reporter gene.
3. Type of genetic modification:
In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
CsVMV promoter: Cassava vein mosaic virus non coding regulatory sequence
Actin1 promoter &first intron: Oryza sativa non coding regulatory sequence
Tubulin intron: Oryza sativa non coding regulatory sequence
FAD2 intron: Arabidopsis thaliana non coding regulatory sequence
bar Streptomyces hygroscopicus coding sequence
nos 3’: Agrobacterium tumefaciens non coding regulatory sequence
sac66 Arabidopsis thaliana non coding regulatory sequence
GFP Aequoria victoria coding sequence
CaMV 3’ Cauliflower mosaic virus non coding regulatory sequence
Transcription factor 1: Zea mays coding sequence
Transcription factor 2: Zea mays coding sequence
Transcription factor 3: Zea mays coding sequence
Transcription factor 4: Zea mays coding sequence
Transcription factor 5: Zea mays coding sequence
Ds 5’ / Ds 3’: Zea mays non coding sequence
RB / LB: Agrobacterium tumefaciens non-coding sequence
6. Brief description of the method used for the genetic modification:
Agrobacterium tumefaciens mediated transformation.
7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
1. Purpose of the release:
Functional gene validation.
Notations and measures of different physiological parameters during the vegetative and flowering stages.
Production of plant and grain under agronomic conditions.
Grain and total biomass production evaluation.
2. Geographical location of the site:
Center of France (Puy-de-Dôme, Auvergne)
3. Size of the site (m2):
1 500 sq mt maximal size for transgenic plants (2007 to 2010, border rows not included), 2 sites max.
4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Second release for these transformation events.
Environmental Impact and Risk Management
Summary of the potential environmental impact from the release of the GMPts:
None of the introduced traits has potential effects to modify the plant persistency in the environment.
There is no wild relatives of corn in Europe and out-crossing are not possible. In this experiment, there is no modification of the direct or indirect interactions between the modified plants and target or non-target organisms. There is no expected effects on human health and on animal health. Plants will not be used as food. There is no short-term possible effect on specific cultivation management and harvesting techniques.
Potential environmental impact considered minimal, given the conditions of the field trial (location, absence of pollen production or field isolation, minimal contacts with fauna and flora, absence of wild relatives of maize species in the release areas...).
Brief description of any measures taken for the management of risks:
Release sites located in intensive agriculture areas. Collection of seeds, destruction of plant vegetative material.
Monitoring and destruction of volunteers during one year following the end of experiment.
Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
European Commission administrative information
Consent given by the Member State Competent Authority: