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Notification report


General information

Notification Number
B/FR/04/05/01

Member State to which the notification was sent
France

Date of acknowledgement from the Member State Competent Authority
21/07/2004

Title of the Project
Evaluation of transgenic grapevines for resistance to Grapevine fanleaf virus and assessment of potential environmental impact

Proposed period of release:
01/05/2004 to 31/12/2008

Name of the Institute(s) or Company(ies)
INRA, 147, rue de l'Université
75338 Paris Cedex 07;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
grape (vinifera x berlandieri)vitaceaevitisvitis vinifera x vitis berlandieri41 B

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The coat protein (CP) gene of Grapevine fanleaf virus (GFLV) and the marker gene Neomycine phosphotransferase II (NPT II) have been introduced in the genome of the grapevine rootstock 41 B.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The coat protein (CP) gene of Grapevine fanleaf virus (GFLV) strain F13 will confer resistance to GFLV and the NPT II gene enables the recovery of transgenic plants upon Agrobacterium-mediated transformation

6. Brief description of the method used for the genetic modification:
Embryogenic cells of the grapevine rootstock 41 B were tranformed using Agrobacterium tumefasciens strain LB4404 harboring the GFLV CP and NPT II genes within the T-DNA borders of pBin19 plasmid. Kanamycine-resistant somatic embryos were selected and developed into plantlets

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
The objective of the release is to evaluate the level of resistance toward GFLV and assess the potential environmental impact of the transgenic grapevine rootstocks

2. Geographical location of the site:
The release site is located on the Huben experimental farm at the INRA Research Center in Colmar, France

3. Size of the site (m2):
The size of the site is 1000 m2 , including 35 m2 established with transgenic grapevines

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
Not applicable

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The major potential environmental impact is related to the possibilities of recombination events between challenging viral RNAs and transgene transcripts

Brief description of any measures taken for the management of risks:
Several measures are taken for risk management.
1. the site is isolated from other grapevine fields
2. a non-transgenic scion will be grafted onto the transgenic rootstock
3. side-shoots will be eliminated from transgenic rootstock, as well as inflorescences from scions
4. a fallowed area and border rows of non transgenic grapevines will enable to control and monitor the spread of the virus and its nematode vector
5. plants will be uprooted and destroyed at the end of the trial and the soil desinfected with nematicides
6. all material collected upon pruning will be autoclaved


Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known