Summary Notification

General information

Notification Number
B/FR/03/03/05

Member State to which the notification was sent
France

Date of acknowledgement from the Member State Competent Authority
17/02/2003

Title of the Project
Field experiment of corn genetically modified for the lignin biosynthesis pathway

Proposed period of release:
01/04/2003 to 30/11/2006

Name of the Institute(s) or Company(ies)
Biogemma, ;

3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)

Common Name	Family Name	Genus	Species	Subspecies	Cultivar/breeding line
maize	poaceae	zea	zea mays	mays	A188 line

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Modification of the lignin composition and content.
Transgenic plants contain the bar gene conferring resistance to glufosinate.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
CsVMV promoter: Cassava vein mosaic virus non coding regulatory sequence
F5H ferulate-5-hydroxylase Zea mays antisense of coding sequence
caffeoyl-coenzymeA
O-methyl transferase Zea mays antisense of coding sequence
3' UTR of peroxydase Zea mays non coding regulatory sequence
Actin1 promoter & first intron Oryza sativa non coding regulatory sequence
bar (pat) Streptomyces hygroscopicus coding sequence
nos 3' Agrobacterium tumefaciens non coding regulatory sequence
RB / LB Agrobacterium tumefaciens non coding sequence

6. Brief description of the method used for the genetic modification:
Agrobacterium tumefaciens mediated transformation.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable

Experimental Release

1. Purpose of the release:
Production of vegative plant material and grain under agronomic conditions.
Analysis of lignin composition and content.

2. Geographical location of the site:
Center of France (Puy-de-Dôme, Auvergne)
South-west of France (Haute-Garonne, Midi-Pyrénées)

3. Size of the site (m2):
Auvergne 1500 m2 (2003 to 2006, border rows not included)
Midi-Pyrénées 1500 m2 (2003 to 2006, border rows not included)

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
First release for these transformation events.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
None of the introdruced traits has potential effects to modify the plant persistency in the environment (plants will not be sprayed with herbicide). There is no wild relatives of corn in Europe and outcrossing is not possible. In this experiment , there is no modification of the direct or indirect interactions between the modified plants and target or non-target organisms. There is no expected effects on human health and on animal health. Plants will not be used as food. There is no short-term possible effect on specific cultivation management and harvesting techniques.
Potential environmental impact is considered minimal, given the conditions on the field trial (location, absence of pollen production, presence of 4 rows of non-transgenic maize plants around the field trial, minimal contacts with fauna and flora, absence of wild relatives of maize species in the release areas ...).

Brief description of any measures taken for the management of risks:
Release sites located in intensive agriculture areas. Collection of seeds and vegetative parts of plants.
Monitoring and destruction of volunteers during one year following the end of experiment.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Not known

Notification report