General informationNotification NumberB/FR/03/01/04Member State to which the notification was sentFranceDate of acknowledgement from the Member State Competent Authority19/12/2002Title of the ProjectAssessment in the field of CMV virus recombination in the presence of tobaccos transformed with a CMV coat protein gene.Proposed period of release:01/05/2003 to 31/10/2003Name of the Institute(s) or Company(ies)Institut du Tabac, ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)| Common Name | Family Name | Genus | Species | Subspecies | Cultivar/breeding line | | tobacco | solanaceae | nicotiana | nicotiana tabacum | tabacum | Xanthi |
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2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:- CP-CMV gene produce CP mRNA
- NptII gene confer kanamycin resistanceGenetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:- R strain of cucumber Mosaic Virus for CP-CMV gene
- Cauliflower Mosaic Virus for 3 promoter
- Pea for Rubisco E. terminator (rbc)
- NptII gene (Tn (E.Coli)) confer kanmycin resistance
- Agrobacterium tumefaciens for Nos promoter and terminator.6. Brief description of the method used for the genetic modification:The chimeric gene was introduced in the tabacco genome by Agrobacterium tumefaciens.Experimental Release1. Purpose of the release:Assessment of the risk associated with the release into the environment of transgenic tobaccos CMV resistant.2. Geographical location of the site:Institut du Tabac - Bergerac (24)3. Size of the site (m2):500 m2Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:No significant impact known todayBrief description of any measures taken for the management of risks:- Separation of 200m grom the genitors
- Avoid flowers by topping
- Destruction by mechanical way after the release.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:Not known