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Notification report


General information

Notification Number
B/FR/03/01/03

Member State to which the notification was sent
France

Date of acknowledgement from the Member State Competent Authority
16/12/2002

Title of the Project
Rhizomania resistant sugar beet France 2003-2007

Proposed period of release:
01/01/2003 to 31/12/2007

Name of the Institute(s) or Company(ies)
Syngenta Seeds SA, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
sugar beetchenopodiaceaebetabeta vulgarisvulgaris var. sacchariferaEvents Rz11, Rz13 and Rz17 and offspring from these events

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
The trait that is introduced is resistance to Rhizomania. Rhizomania is a disease in sugar beet that is spreading rapidly. The gene used for the resistance trait is from the virus that confers the disease, Beet Necrotic Yellow Vein Vrius (BNYVV).
As selectable marker was phosphomannose isomerase (PMI) used. PMI allows for the positive selection of cells that express the PMI gene.


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Two genes were introduced into the sugar beet,
1) Rzm, the gene that confers Rhizomania resistance, and
2) PMI, the gene that allows for a selection of transformed cells in tissue culture.
The sugar beet was transformed with a plasmid carrying two genes:
- a gene that confers resistance to Rhizomania
- a gene that allows selection on media containing mannose
See table below for details

DNA sequence Size of sequence Intended function Source and reference
Ubiquitin 3 1,3 kb Promoter Arabidopsis thaliana
RZM 1,6 kb Resistance to BNYVV BNYVV, Bouzoubaa et al., 1987
Nos 0,3 kb Terminator Agrobacterium tumefaciens, Fraley et al., 1983
Hsp80 1,5 kb Promoter Brassica sp., Brunke & Wilson 1993
PMI 1,2 kb Selectable marker E. coli, Joersbo et al., 1998
35S 0,2 kb Terminator Cauliflower Mosaic Virus, Odell et al., 1985


6. Brief description of the method used for the genetic modification:
The sugar beet was transformed using standard Agrobacterium mediated transformation techniques.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not relevant

Experimental Release

1. Purpose of the release:
The purpose of the release is to evaluate the level of resistance to BNYVV.

2. Geographical location of the site:
The release will take place in France in Courcelles (45300) in the area of Pithiviers.

3. Size of the site (m2):
The site of release will not exceed 800 m2. This area includes the GM plants, the non-GM control plants, and the surrounding area that is not cultivated.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
The same events were grown in a deliberate release in the US in 2002. No negative impact on the environment or human health were recorded during this release.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The GM sugar beet will not be allowed to bolt. This will eliminate the risk of pollen spread of the GM trait.
The site will be monitored for one year after the release. During the year after the release, the site will be grown with a monocot species. Any volunteer sugar beet appearing in the monocot crop, will be eliminated by the herbicides used in monocot crops, or removed by mechanical means.
Based on the above, it is our conclusion that this release will have negligible impact on the environment.


Brief description of any measures taken for the management of risks:
The GM sugar beet will not be allowed to bolt. This will eliminate the risk of pollen spread of the GM trait.
The site will be monitored for one year after the release. During the year after the release, the site will be grown with a monocot species. Any volunteer sugar beet appearing in the monocot crop, will be eliminated by the herbicides used in monocot crops, or removed by mechanical means.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
The purpose of this release is to evaluate the level of resistance to BNYVV, not to gain new data on the environmental and human health impact of the release.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known