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Notification report


General information

Notification Number
B/ES/17/03

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
23/01/2017

Title of the Project
Use of plastidial Glucose 6P dehydrogenase for production of potato plants with increased starch content.

Proposed period of release:
01/05/2017 to 30/09/2017

Name of the Institute(s) or Company(ies)
Instituto de Agrobiotecnología,
Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):
B/ES/15/02;

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosum

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
B33-StG6PDH-as potato plants produced have a complete tuber G6PDH cDNA in antisense direction under the control of B33-promoter. In this construction the selection gen was Hyg (Hygromycin resistance).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Gen: platidial G6PDH (cDNA from potato, 1734 pb in antisense direction).
Selection Gen: Hyg (Hygromycin resistance).
Promoter Gen: B33 (potato tuber patatin promoter).


6. Brief description of the method used for the genetic modification:
Potato plants transformation was conducted using Agrobacterium tumefaciens strain.
C58C1:GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U.,
Frommer W.B., Starmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29)


Experimental Release

1. Purpose of the release:
B33-StG6PDH-as plants grown in green-house exhibited an increase in starch content as compare with WT, reduced glucose and fructose contents and no changes in tuber yield and sucrose and maltose content could be detected. In order to prove that these results also occur in field grown potato plants we decided to release this material. If results are as expected, these plants could be of great agronomic interest (will allow to reduce field area and agrochemicals and to produce bioenergy).

2. Geographical location of the site:
Genetic modified potato plants will be released in Tudela (Navarra-Spain). The experimental Field, which belongs to the Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias (INTIA), a governmental Institution, is located in a dry area (5-50 mm precipitation) in south Navarra.

3. Size of the site (m2):
The experimental Filed has 20 ha. However we will be using only 183 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
We have observed no adverse effects of the transgenic lines on environment during the release realized in 2015 (B/ES/15/02).
All the weeds and pets (potato beetle) affected in the same way to the border and the experimental lines (Desiree and OMGs) and were typical for corn.
We have either observed any adverse effect on human health. No one of us (from the research Institute and the experimental field) had any health problem.


Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced traits- expression of potato tuber G6PDH under the control patatin B33 promoter- are not expected to confer any selective advantage either any effect on environment when compared with control plants.

Brief description of any measures taken for the management of risks:
- We will control that nobody else than us or the personal working in the experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants.
- All the vegetal material that is not going to be used will be destroyed, by autoclaving.
- Potato tubers will not be seeded on the same parcel the following campaign.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We will plant 300 potato tubers. We will work with 4 genetic modified lines and the control plants (WT). We will use 60 tubers per line, divided in 3 sub-parcels of 20 tubers each.

Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known