General informationNotification NumberB/ES/15/02Member State to which the notification was sentSpainDate of acknowledgement from the Member State Competent Authority08/01/2015Title of the ProjectUse of plastidial Glucose 6P dehydrogenase for production of potato plants with increased starch contentProposed period of release:01/04/2015 to 01/09/2015Name of the Institute(s) or Company(ies)Instituto de Agrobiotecnología, Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:B33-StG6PDH-as potato plants produced have a complete tuber G6PDH cDNA in antisense direction under the control of B33-promoter. In this construction the selection gen was Hyg (Hygromycin resistance).Genetic modification3. Type of genetic modification:Insertion; Other; OtherpH2-B33-StG6PDH-asIn case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:Gen: platidial G6PDH (cDNA from potato, 1734 pb in antisense direction)
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
Selection Gen: Hyg (Hygromycin resistance)
Promoter Gen: B33 (potato tuber patatin promoter)6. Brief description of the method used for the genetic modification:Potato plants transformation was conducted using Agrobacterium tumefaciens strain C58C1:GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U., Frommer W.B., Starmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29)Experimental Release1. Purpose of the release:B33-StG6PDH-as plants grown in green-house exhibited an increase in starch content as compare with WT, a reduce in glucose and fructose contents and no changes in tuber yield and sucrose and maltose content could be detected. In order to prove that these results also occur in field grown potato plants we decided to release this material. If results are as expected, these plants could be of great agronomic interest (will allow to reduce field area and agrochemicals and to produce bioenergy).2. Geographical location of the site:Genetic modified potato plants will be released in Sartaguda (Navarra-Spain). The experimental Field, which belongs to the Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias (INTIA), a governmental Institution, is located in a dry area (5-50 mm precipitation)in south Navarra.3. Size of the site (m2):The experimental Filed has 19 ha. However we will be using only 232 m2.4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:There is not previous releases.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The introduced traits- expression of potato tuber G6PDH under the control patatin B33 promoter- are not expected to confer any selective advantage either any effect on environment when compared with control plants.Brief description of any measures taken for the management of risks:- We will control that nobody else than us or the personal working in the experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants.
- All the vegetal material that is not going to be used will be destroyed, by autoclaving.
- Potato tubers will not be seeded on the same parcel the following campaign.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:We will plant 450 potato tubers. We will work with 4 genetic modified lines and the control plants (WT). We will use 90 tubers per line, divided in 3 sub-parcel of 30 tubers each.Final reportEuropean Commission administrative informationConsent given by the Member State Competent Authority:Yes27/05/2015 00:00:00Remarks:Authorized by the Autonomous Community of Navarra.