General informationNotification NumberB/ES/15/01Member State to which the notification was sentSpainDate of acknowledgement from the Member State Competent Authority26/01/2015Title of the ProjectUsing transgenic plants plum as rootstocks of commercial varieties of peach and apricot.Proposed period of release:01/03/2015 to 31/12/2018Name of the Institute(s) or Company(ies)Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC)., ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:The strain EHA105, carrying the binary vector pCGN1578 was used for inoculation of hypocotyl slices as described in Petri et al., (2008). The cytosolic apx1 (cytapx) cDNA from Pisum sativum with a size of 1054 bp (Gen-Bank accession number X62077), was obtained from the plasmid pRTL2, removed by HindIII digestion, and cloned in the HindIII site of pCGN1578. The resulting construction also harboured in the T-DNA the neomycin phosphotransferase (nptII) gene for aminoglycoside selection by adding kanamycin to the culture media.
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
|plum||rosaceae||prunus||prunus domestica||Claudia Verde|
The cytapx transgene is under the control of the duplicated CaMV35S promoter plus TEV enhancer and Nos terminator.
J8-1 line has four copies of the T-DNA. The role of the cytosolic protein APX is to scavenge H2O2. Under in vitro conditions, cytapx gene expression levels are about 900 times higher in transgenic plants than untransformed plants. APX activity levels in the transformed plants are 20% and 40% higher under in vitro and ex vitro conditions respectively than in non-transformed plants.Genetic modification3. Type of genetic modification:Insertion; In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:The T-DNA used contains between the left and right edges an nptII gene conferring resistance to aminoglycoside antibiotics under the control of 35S promoter and terminator. Moreover, cytapx gene was cloned under the control of a 2x35S promoter and the untranslated region of the large tumors morphology from Agrobacterium tumefaciens as terminator (3'tml).6. Brief description of the method used for the genetic modification:We used the transformation protocol described by Petri et al. (Mol Breed 2008; 22: 581-91) for European plum hypocotyls mediated by Agrobacterium tumefaciens.7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:It is a fruit tree but given that the intention of the project is to evaluate their behavior as rootstock there is no risk of spread.Experimental Release1. Purpose of the release:We aim to investigate the behavior of the GMHP (Genetically Modified Higher Plants) as a rootstock for different commercial varieties of Prunus under the regular cropping and environmental conditions of Murcia. These GMHP are more vigorous and have shown to be tolerant to water stress under greenhouse conditions. In addition, due to the low rainfall in the areas under study, the use of these plants as rootstock could increase production of commercial varieties tested.
The GMHPs will be released in cropping plots with fruit trees belonging to the same genus. No invasion of natural habitats and no gene transfer are expected, since GMHPs will not develop reproductive organs, and that will be used only as a rootstock.
The interaction with other living organisms is minimal, and common control measures will be applied in order to prevent infection by fungi, bacteria and plant viruses. Furthermore, the interaction of both humans and natural predators with these crops is basically due to the intake of fruits, which will be not produced in any case for the GMHPs. Finally, interaction between the GMHPs and soil microorganisms has not been described.2. Geographical location of the site:The release site is located in three areas:
1. Archena (Murcia).
2. Campos del Rio (Murcia).
3. Villanueva del Rio Segura (Murcia).3. Size of the site (m2):Area 1: 2800 m2
Area 2: 6520000 m2
Area 3: 12410000 m24. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:There have been no previous releases.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The GMHPs has been shown to be more tolerant to salinity (up to 150 mM NaCl, Diaz-Vivancos et al., 2013, Plant Biotech J, 11, 976-985) under in vitro conditions, and more tolerant to water stress under greenhouse conditions (Diaz-Vivancos et al., Plant Biotech J 2014, in review) than untransformed plants.Brief description of any measures taken for the management of risks:There will be no risk of dispersion because the GMHPs will be used as rootstock and reproductive organs will be developed. The experimental plots will be visited regularly for both the research team and the farmers, limiting the development of the vegetative part of the GMHPs by pruning them. The pruning’s will be autoclaved and disposed CEBAS-CSIC facilities.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:Yes12/05/2015 00:00:00Remarks: