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Notification report


General information

Notification Number
B/ES/14/01

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
16/01/2014

Title of the Project
Use of hexose phosphate translocator for production of potato plants with increased starch content

Proposed period of release:
01/04/2014 to 30/09/2015

Name of the Institute(s) or Company(ies)
Instituto de Agrobiotecnología,
Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumDesirée

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
GPT2 overexpressing potato plants produced have a complete potato GPT2 cDNA under the control of CaMV-35S-promoter. In this construction the selection gen was NPTII (Kanamycin resistance).

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Gen: GPT2 (complete glucose 6P translocator cDNA from potato, 1206 pb)
Selection Gen: Kan (NPTII gen, kanamycin resistance)
Promoter Gen: CaMV-35S (cauliflower mosaic virus promoter)
Terminator Gen: Nos (Agrobacterium nopaline synthase polyadenilation gen)


6. Brief description of the method used for the genetic modification:
Potato plants transformation was conducted using Agrobacterium tumefaciens strain C58C1:GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U.,Frommer W.B., Starmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29)

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
-

Experimental Release

1. Purpose of the release:
GPT2 overexpressing plants grown in green-house exhibited an increase in starch
content as compare with WT and no changes in tuber yield and soluble sugars content could be detected. In order to prove that these results also occur in field grown potato plants we decided to release this material. If results are as expected,these plants could be of great agronomic interest (will allow to reduce filed area and agrochemicals and to produce bioenergy)


2. Geographical location of the site:
Genetic modified potato plants will be released in Sartaguda (Navarra-Spain). The experimental Field, which belongs to the Instituto Navarro de Tecnologías e
Infraestructuras Agroalimentarias (INTIA), a governmental Institution, is located in a dry area (5-50 mm precipitation) in south Navarra


3. Size of the site (m2):
The experimental Filed has 19 ha. However we will be using only 232 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
There is not previous releases.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced traits- overexpression of potato GPT2 under the control of CaMV-
35S promoter- are not expected to confer any selective advantage either any effect on environment when compared with control plants.


Brief description of any measures taken for the management of risks:
- We will control that nobody else than us or the personal working in the
experimental filed harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants
- All the vegetal material that is not going to be used will be destroyed, by
autoclaving
- Potato tubers will not be seeded on the same parcel the following campaign


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We will plant 450 potato tubers. We will work with 4 genetic modified lines and the control plants (WT). We will use 90 tubers per line, divided in 3 sub-parcel of 30 tubers each.

Final report
-

European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
09/05/2014 00:00:00
Remarks:
Authorized by the Autonomous Community of Navarra.