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Notification report


General information

Notification Number
B/ES/10/50

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
31/05/2010

Title of the Project
Field test of Nicotiana glauca genetically modified as an energy crop

Proposed period of release:
01/06/2010 to 01/06/2011

Name of the Institute(s) or Company(ies)
ABBA Gaia, S.L., ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
Tree tobaccoSolanaceaeNicotianaNicotiana glauca

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Gene TaPCS1 (gene encoding wheat phytochelatin synthase) was inserted into the genome of Nicotiana glauca under the control of the 35S constitutive promoter of cauliflower mosaic virus.

Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
The genetic material inserted into Nicotiana glauca is composed of a phytochelatin synthase gene of wheat under the control of 35S promoter of cauliflower mosaic virus that provides phytochelatin synthase and phytochelatins overexpression in the plant, and the gene NptII (neomycin phosphotransferase gene) that confers resistance to the antibiotic kanamycin for selection of transformed plants.

The insert length is 1503 bp. This insert was introduced in the binary vector pBI121 (14.758 bp).

The host range of the vector is the bacteria E. coli and any plant capable of being infected by Agrobacterium tumefaciens.

This genetic modification is performed in Nicotiana glauca targeting on its natural ability to absorb heavy metals and other contaminants from the soil. This modification also provides a higher biomass production.


6. Brief description of the method used for the genetic modification:
The implemented method for genetic modification is infection with Agrobacterium tumefaciens.

Experimental Release

1. Purpose of the release:
Te goal of this release is conducting a trial for evaluation of the genetically modified line for biomass production under different cultivation frames.

2. Geographical location of the site:
Benicàssim (Castellón).

3. Size of the site (m2):
This is a plot of agricultural land in an area of 43.796 m2.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The wild plant Nicotiana glauca grows very selectively in places that have supported human activity (empty lots, landfills, dumps, etc.). This plant hasn’t an uncontrolled spread in environments where other native plants grow naturally, so this plant (both genetically modified and wild type species) contributes to restore those places.

On the other hand, this plant has a natural capacity of soil decontamination by absorbing heavy metals and other contaminants. There are studies demonstrating the capacity of this genetically modified plant, to absorb soil pollutants and to increase biomass production.

This plant, both the wild and the GMHP, not intended for human nor animal consumption.


Brief description of any measures taken for the management of risks:
- The method of release of genetically modified plants is: after germination in a seedbed under greenhouse conditions, 10 cm-height plants are transplanted to the cultivation field.

- The genetically modified Nicotiana glauca will be cultivated in the middle of the field. Also, as part of the experience, wild type Ng plants will be cultivated around the perimeter of the cultivation area as a barrier-shelter to prevent the spread of the modified species. So the genetically modified species are confined to the central area of the trial-field.

- Seeds will be collected for further oil extraction studies and energy evaluation.

- Nicotiana glauca is an autogamous plant, so the probability for cross-fecundation is very small based on bibliographic data and experiences in own greenhouses.

- Once the plants are harvested (both GMHP as the wild plant used as a barrier-shelter) the trial-field will be treated properly to prevent further growth of the plant from any remainder sprouts stayed on the ground (treatments with herbicides etc.).

- Regular tests will be conducted in the surrounding areas in order to ensure no proliferation of plants out of the trial-field.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
- The method of release of genetically modified plants is: after germination in a seedbed under greenhouse conditions, 10 cm-height plants are transplanted to the cultivation field.

- The genetically modified Nicotiana glauca will be cultivated in the middle of the field. Also, as part of the experience, wild type Ng plants will be cultivated around the perimeter of the cultivation area as a barrier-shelter to prevent the spread of the modified species. So the genetically modified species are confined to the central area of the trial-field.

- Seeds will be collected for further oil extraction studies and energy evaluation.

- Nicotiana glauca is an autogamous plant, so the probability for cross-fecundation is very small based on bibliographic data and experiences in own greenhouses.

- Once the plants are harvested (both GMHP as the wild plant used as a barrier-shelter) the trial-field will be treated properly to prevent further growth of the plant from any remainder sprouts stayed on the ground (treatments with herbicides etc.).

- Regular tests will be conducted in the surrounding areas in order to ensure no proliferation of plants out of the trial-field.


Final report
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European Commission administrative information

Consent given by the Member State Competent Authority:
Not known