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Notification report


General information

Notification Number
B/ES/10/43

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
25/01/2010

Title of the Project
Starch synthase (AtSS4) use for the production of potato plants with increased starch levels.

Proposed period of release:
01/03/2010 to 30/09/2010

Name of the Institute(s) or Company(ies)
Instituto de Agrobiotecnología,
Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumDesiree

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Starch synthase overexpressing potato plants produced have a complete Arabidopsis thaliana SS4 cDNA under the control of CaMV-35S-promoter . In this construction the selection gen was NPTII (kanamicin resistance).

Genetic modification

3. Type of genetic modification:
Insertion; Other;
Other
p35S-AtSS4-Nos

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
- Gen: Starch synthase (complete SS4 cDNA from Arabidopsis thaliana, 3123bp).
- Selection gen: Kanamicin resistance (gen NPTII).
- Promotor genes: CaMV35S (cauliflower mosaic virus promoter).
- Terminator: NOS (nopaline synthase gen from Agrobacterium).


6. Brief description of the method used for the genetic modification:
Potato plants transformation was conducted using Agrobacterium tumefaciens strain C58C1:
GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U., Frommer W.B., Startmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29).


Experimental Release

1. Purpose of the release:
Starch synthase over-expressing plants grown in green-house and in filed (B/ES/09/50) exhibited an increase of starch levels as compare with WT and no pleiotrophic changes in photosynthetic parameters and maximun catalytic activities of enzymes closely linked to starch metabolism could be detected. In order to confirm these results in field grown potato plants we decided to release
again this material. If results are as expected, these plants could be of great agonomic interest (will allow to reduce field area and to produce agroenergy).


2. Geographical location of the site:
Genetic modified potato plants will be released in Sartaguda (Navarra-Spain). The Experimental Field, which belongs to the Instituto Técnico de Gestión Agrícola (ITGA), a governmental Institution, is located in a dry area (5-50 mm precipitation) in south Navarra.

3. Size of the site (m2):
The experimental field has 19 ha. However we will be using only 120 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
We have observed no adverse effects of the transgenic lines on environment during the release realized in 2009 (B/ES/09/50).
All the weeds and pets detected affected in the same way to the WT and the transgenic lines and were typical for potato.
We have either observed any adverse effect on human health. No one of us (form the Institutte or the Experimentall Filed) have had any health problem.


Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced traits- overexpression of Sucrose synthase gen under the control of CaMV-35S promoter- are not expected to confer any selective advantage either any effect on environment when compared with control plants.

Brief description of any measures taken for the management of risks:
- We will control that nobody other people´s to the experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants.
- It will be destroyed , by autoclaving, all the vegetal material that is not going to be used.
- Potato tubers will not be seeded on the same parcel the following campaign.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We will plant 450 potato tubers. We will work with 4 genetic modified lines and the control plants (WT line). We will use 90 tubers of each line, divided in 3 subparcel of 30 tubers each.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
12/05/2010 00:00:00
Remarks:
The competent authority for give the consent of these field trials is the Autonomous Community of Navarra.