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Notification report


General information

Notification Number
B/ES/10/40

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
11/03/2010

Title of the Project
Study of maize lignification for the improvement of digestibility and bioethanol production.

Proposed period of release:
01/04/2010 to 31/10/2010

Name of the Institute(s) or Company(ies)
Idén Biotechnology S.L., ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
maizepoaceaezeazea maysmaysA188 X B73

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
Transgenic maize plants produced have the insert CAD-RNAi under the control of the ubiquitine promoter.
In this construction the selection gene was BAR (BASTA resistance).


Genetic modification

3. Type of genetic modification:
Insertion; Other;
Other
plasmid pAHC25, which includes the insert CAD-RNAi and the BAR gene, both under the ubiquitine promoter control.

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
Gene: the interest fragment of 1,5 kb includes the first exon of the CAD maize gene (Cinnamyl Alcohol Dehydrogenase), followed by the first intron and the same region of the first exon oriented in antiparallel to obtained an interference RNA denominated CAD-RNAi.

Selection gene: BAR (herbicide BASTA resistance).

Gene promotor: Ubi-1 (maize ubiquitine promoter).


6. Brief description of the method used for the genetic modification:
The method used to obtain the transgenic plants has been the biolistic method.

Experimental Release

1. Purpose of the release:
CAD-RNAi expressing plants have inactivated the enzyme CAD which is implicated in the lignin production in the plant. The presence of lignin in the cell wall makes the extraction of cell wall polysacharides for its convertion to bioethanol difficult.
CAD-RNAi expressing plants grown in green-house exhibited an altered lignin production and an increase in its degradability for the production of bioethanol celulosic in vitro as compared with WT. In order to prove that these results also occur in field grown maize plants, we decided to release this material. If results are as expected, these plants could be of great agronomic interest related to the obtention of bioethanol by using the residual biomass of the maize plants.


2. Geographical location of the site:
Genetic modified maize plants will be released in Santesteban (Navarra-Spain). The Experimental Field, which belongs to the Instituto Técnico Agrícola (ITGa), a governmental Institution, is located in the north of Navarra.

3. Size of the site (m2):
Approximately 206,8m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
There are not previous releases.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced traits are not expected to confer any selective advantage either any effect on environment when compared with control plants.

Brief description of any measures taken for the management of risks:
-We will control that only authorized staff gain access to the experimental field.
-The maize plants will be surrounded by sexually remote plants.
-It will be destroyed, by autoclaving, all the vegetal material that is not going to be used for investigations purposes.
-Maize will not be seeded on the same parcel the following campaign.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We will plant 144 maize seeds.
We will work with a genetic modified line and the control plants (WT line).
We will use 72 seeds of each line, divided in 3 sub parcels of 24 seeds each.


Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Yes
12/05/2010 00:00:00
Remarks:
The competent authority for give the consent of these field trials is the Autonomous Community of Navarra.