Back

Notification report


General information

Notification Number
B/ES/08/45

Member State to which the notification was sent
Spain

Date of acknowledgement from the Member State Competent Authority
23/04/2008

Title of the Project
Glycogen phosphorylase use for production of potato plants with increased starch
levels.


Proposed period of release:
01/06/2008 to 30/09/2008

Name of the Institute(s) or Company(ies)
Instituto de Agrobiotecnología,
Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;


3. Is the same GMPt release planned elsewhere in the Community?
No

Has the same GMPt been notified elsewhere by the same notifier?
No

Genetically modified plant

Complete name of the recipient or parental plant(s)
Common NameFamily NameGenusSpeciesSubspeciesCultivar/breeding line
potatosolanaceaesolanumsolanum tuberosumtuberosumDesirée

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
glgP overexpressing potato plants produced have a complete glgP cDNA under the control of B33-promoter. In this construction the selection gen was NPTII (kanamicin resistance).

Genetic modification

3. Type of genetic modification:
Insertion; Other;
Other
pBINB33-ChlTP-glgP-NOS

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
- Gen: Glycogen phosphorylase (complete glgP cDNA from the non patogenic Escherichia coli BL21 (DE3)).
- Selection gen: Kanamicin resistance (gen NPTII).
- Promotor genes: B33(patatin gene promoter).
- Transit peptide: ChlTP (transit peptide of the Capsicum annun P541 protein).
- Terminator: NOS (nopaline synthase gen from Agrobacterium).


6. Brief description of the method used for the genetic modification:
Potato plants transformation was conducted using Agrobacterium tumefaciens strain C58C1: GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U., Frommer W.B., Startmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29).

Experimental Release

1. Purpose of the release:
glgP over-expressing plants grown in green-house exhibited a large increase of starch levels and diferent starch composition as compare with WT . In order to prove that these results also occur in field grown potato plants, we decided to release this material. If results are as expected, these plants could be of great agonomic interest (will allow to reduce field area and to produce agroenergy).

2. Geographical location of the site:
Genetic modified potato plants will be released in Sartaguda (Navarra-Spain). The Experimental Field, which belongs to the Instituto Técnico de Gestión Agrícola (ITGA), a governmental Institution, is located in a dry area (5-50 mm precipitation) in south Navarra.

3. Size of the site (m2):
The experimental field has 19 ha. However we will be using only 100 m2.

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
There is not previous releases.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The introduced traits-overexpression of SGlycogen phosphorylase gene under the control of B33 promoter-are not expected to confer any selective advantage either any effect on environment when compared with control plants.

Brief description of any measures taken for the management of risks:
- We will control that nobody other people´s to the experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants.
- It will be destroyed , by autoclaving, all the vegetal material that is not going to be used.
- Potato tubers will not be seeded on the same parcel the following campaign.


Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
We will plant 420 potato tubers. We will work with 6 genetic modified lines and the control plants (WT line). We will use 60 tubers of each line, divided in 3 subparcel of 20 tubers each.

Final report


European Commission administrative information

Consent given by the Member State Competent Authority:
Not known