General informationNotification NumberB/ES/05/23Member State to which the notification was sentSpainDate of acknowledgement from the Member State Competent Authority03/06/2005Title of the ProjectSucrose synthase characterization and its use for production of potato plants with increased starch levels.Proposed period of release:01/06/2005 to 30/09/2005Name of the Institute(s) or Company(ies)Instituto de Agrobiotecnología,
Universidad Pública de Navarra/Consejo Superior de Investigaciones Científicas, ;
3. Is the same GMPt release planned elsewhere in the Community?NoHas the same GMPt been notified elsewhere by the same notifier?NoGenetically modified plantComplete name of the recipient or parental plant(s)
2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:SuSy overexpressing potato plants produced have a complete potato Sus4 cDNA under the control of CaMV-35S-promoter . In this construction the selection gen was NPTII (kanamicin resistance).Genetic modification3. Type of genetic modification:Insertion; Other; OtherpBIN35S-susy-NosIn case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:Gen: Sucrose synthase (complete Sus4 cDNA from potato, 2425bp, accession number AJ537575 )
|Common Name||Family Name||Genus||Species||Subspecies||Cultivar/breeding line|
Selection gen: Kanamicin resistance (gen NPTII)
Promotor genes: CaMV35S (cauliflower mosaic virus promoter)
Terminator: NOS (nopaline synthase gen from Agrobacterium)6. Brief description of the method used for the genetic modification:Potato plants transformation was conducted using Agrobacterium tumefaciens strain C58C1:GV2260 as described by Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U., Frommer W.B., Startmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29).Experimental Release1. Purpose of the release:SuSy over-expressing plants grown in green-house exhibited a large increase of starch levels as compare with WT and no pleiotrophic changes in photosynthetic parameters and maximun catalytic activities of enzymes closely linked to starch metabolism could be detected. In order to prove that these results also occur in field grown potato plants we decided to release this material. If results are as expected, these plants could be of great agonomic interest (will allow to reduce field area and to produce agroenergy).2. Geographical location of the site:Genetic modified potato plants will be released in Sartaguda (Navarra-Spain). The Experimental Field, which belongs to the Instituto Técnico de Gestión Agrícola (ITGA), a governmental Institution, is located in a dry area (5-50 mm precipitation) in south Navarra.3. Size of the site (m2):The experimental field has 19 ha. However we will be using only 100 m2.4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:There is not previous releases.Environmental Impact and Risk ManagementSummary of the potential environmental impact from the release of the GMPts:The introduced traits- overexpression of Sucrose synthase gen under the control of CaMV-35S promoter- are not expected to confer any selective advantage either any effect on environment when compared with control plants.Brief description of any measures taken for the management of risks:- We will control that nobody other people´s to the experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote plants.
- It will be destroyed , by autoclaving, all the vegetal material that is not going to be used.
- Potato tubers will not be seeded on the same parcel the following campaign.Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:We will plant 420 potato tubers. We will work with 6 genetic modified lines and the control plants (WT line). We will use 60 tubers of each line, divided in 3 subparcel of 20 tubers each.Final report-European Commission administrative informationConsent given by the Member State Competent Authority:No04/08/2005 00:00:00Remarks:This field trial has been retired by the notifier. It will be postpone until the next year.